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Inositol Polyphosphate Phosphatidylinositol 5-Phosphatase9 (At5PTase9) Controls Plant Salt Tolerance by Regulating Endocytosis 被引量:5

Inositol Polyphosphate Phosphatidylinositol 5-Phosphatase9 (At5PTase9) Controls Plant Salt Tolerance by Regulating Endocytosis
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摘要 Phosphatidylinositol 5-phosphatases (5PTases) components of membrane trafficking system. Recently, we that hydrolyze the 5' position of the inositol ring are key reported that mutation in AtSPTase7 gene reduced produc- tion of reactive oxygen species (ROS) and decreased expression of stress-responsive genes, resulting in increased salt sensitivity. Here, we describe an even more salt-sensitive 5ptase mutant, At5ptase9, which also hydrolyzes the 5' phos- phate groups specifically from membrane-bound phosphatidylinositides. Interestingly, the mutants were more tolerant to osmotic stress. We analyzed the main cellular processes that may be affected by the mutation, such as production of ROS, influx of calcium, and induction of salt-response genes. The At5ptase9 mutants showed reduced ROS produc- tion and Ca2~ influx, as well as decreased fluid-phase endocytosis. Inhibition of endocytosis by phenylarsine oxide or Tyrphostin A23 in wild-type plants blocked these responses. Induction of salt-responsive genes in wild-type plants was also suppressed by the endocytosis inhibitors. Thus, inhibition of endocytosis in wild-type plants mimicked the salt stress responses, observed in the AtSptase9 mutants. In summary, our results show a key non-redundant role of At5PTase7 and 9 isozymes, and underscore the localization of membrane-bound Ptdlns in regulating plant salt tolerance by coordinating the endocytosis, ROS production, Ca2+ influx, and induction of stress-responsive genes. Phosphatidylinositol 5-phosphatases (5PTases) components of membrane trafficking system. Recently, we that hydrolyze the 5' position of the inositol ring are key reported that mutation in AtSPTase7 gene reduced produc- tion of reactive oxygen species (ROS) and decreased expression of stress-responsive genes, resulting in increased salt sensitivity. Here, we describe an even more salt-sensitive 5ptase mutant, At5ptase9, which also hydrolyzes the 5' phos- phate groups specifically from membrane-bound phosphatidylinositides. Interestingly, the mutants were more tolerant to osmotic stress. We analyzed the main cellular processes that may be affected by the mutation, such as production of ROS, influx of calcium, and induction of salt-response genes. The At5ptase9 mutants showed reduced ROS produc- tion and Ca2~ influx, as well as decreased fluid-phase endocytosis. Inhibition of endocytosis by phenylarsine oxide or Tyrphostin A23 in wild-type plants blocked these responses. Induction of salt-responsive genes in wild-type plants was also suppressed by the endocytosis inhibitors. Thus, inhibition of endocytosis in wild-type plants mimicked the salt stress responses, observed in the AtSptase9 mutants. In summary, our results show a key non-redundant role of At5PTase7 and 9 isozymes, and underscore the localization of membrane-bound Ptdlns in regulating plant salt tolerance by coordinating the endocytosis, ROS production, Ca2+ influx, and induction of stress-responsive genes.
出处 《Molecular Plant》 SCIE CAS CSCD 2013年第6期1781-1794,共14页 分子植物(英文版)
关键词 abiotic/environmental stress oxidative and photo-oxidative stress SALINITY protein traffic and secretion gene expression Arabidopsis. abiotic/environmental stress oxidative and photo-oxidative stress salinity protein traffic and secretion gene expression Arabidopsis.
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  • 1Allen, G.J., Kuchitsu, K., Chu, S.P., Murata, Y., and Schroeder, J.I. (1999). Arabidopsis abi1-1 and abi2-1 phosphatase mutations reduce abscisic acid-induced cytoplasmic calcium rises in guard cells. Plant Cell. 11,1785-1798.
  • 2Amtmann, A., and Sanders, D. (1999). Mechanisms of Na+ uptake by plant cells. Adv.Bot. Res. 29, 75-112.
  • 3Aniento, F., and Robinson, D.G. (2005). Testing for endocytosis in plants. Protoplasma. 226, 3-11.
  • 4Apel, K., and Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant BioI. 55, 373-399.
  • 5Astle, M., Horan, K., Ooms, l., and Mitchell, C (2007). The inositol polyphosphate 5-phosphatases: traffic controllers, waistline watchers and tumour suppressors? Biochem. Soc. Symp. 074, 161-181.
  • 6Audhya, A., and Emr, S.D. (2003). Regulation of PI4,5P2 synthesis by nuclear-cytoplasmic shuttling of the Mss4 lipid kinase. EMBO J. 22, 4223-4236. Babior, B.M. (2004). NADPH oxidase. Curr. Opin. Immunol. 16, 42-47.
  • 7Berdy, 5., Kudla, J., Gruissem, W., and Gillaspy, G. (2001). Molecular characterization of At5PTase1, an inositol phosphatase capable of terminating IP3 signaling. Plant Physiol. 126, 801-810.
  • 8Bolte,S., Talbot, C, Boutte, Y., Catrice, 0., Read, N.D., and SatiatJeunemaitre, B. (2004). FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells. J. Microsc. 214, 159-173.
  • 9Carland, F.M., and Nelson, T. (2004). COTYLEDON VASCULAR PATIERN2-mediated inositol (1.4,5) triphosphate signal transduction is essential for closed venation patterns of Arabidopsis foliar organs. Plant Cell. 16,1263-1275.
  • 10Carol, RJ., and Dolan, L. (2006). The role of reactive oxygen species in cell growth: lessons from root hairs. J. Exp. Bot. 57,1829-1834.

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