摘要
目的:检测非小细胞肺癌(NSCLC)组织中Nanog的表达。方法:采用免疫组化及实时荧光定量PCR法分别检测106例NSCLC及10例正常肺组织中Nanog蛋白及mRNA的表达,采用生存分析探讨Nanog蛋白的表达与肺癌患者预后的关系。结果:NSCLC组织中Nanog蛋白的阳性表达率为24.5%(26/106),高于正常肺组织(0%,P=0.114);Nanog蛋白在肺腺癌中的表达明显高于肺鳞状细胞癌(肺鳞癌)(χ2=5.087,P=0.024),随病理级别增加,Nanog蛋白的阳性表达率升高(χ2=6.845,P=0.033);Nanog蛋白的表达与肺鳞癌患者的预后无关(χ2=0.013,P=0.917),肺腺癌患者中Nanog阴性表达者预后较好(χ2=5.352,P=0.020)。NSCLC组织中Nanog mRNA的表达为(0.950±0.183),高于正常肺组织的(0.277±0.190)(F=122.662,P<0.001);随病理级别增加,Nanog mRNA的表达升高(F=4.047,P=0.020)。结论:Nanog的表达与NSCLC的发生发展有关,且Nanog蛋白的表达与肺腺癌的预后有关。
Aim：To investigate the expression of Nanog in non-small cell lung cancer（ NSCLC） tissue. Methods：The expressions of Nanog mRNA and protein were detected in the specimens from 106 cases of NSCLC and 10 cases of normal lung tissue by using quantitative real-time PCR and immunohistochemistry,respectively,then the relationship between the expression of Nanog protein and the prognosis of the patients with lung adenocarcinoma（ LAC） or lung squmous cell carcinoma（ LSCC） was analyzed by log-rank test. Results：The positive expression rate of Nanog protein in NSCLC tissue was24. 5%,higher than that（ 0%） in normal lung tissue（ P = 0. 114）; the positive expression rate of Nanog protein was higher in LAC tissue than that in LSCC tissue and increased with the increase of pathological degree（ χ2= 5. 087,6. 845,P 0. 05）; in patients with LAC,those with negative expression of Nanog protein had better progonosis（ χ2= 5. 352,P =0. 020）. The expression level of Nanog mRNA in NSCLC tissue was（ 0. 950 ± 0. 183）,higher than that（ 0. 277 ± 0. 190）in normal lung tissue（ F = 122. 662,P 0. 001）; the expression level of Nanog mRNA increased with the increase of pathological degree（ F = 4. 047,P = 0. 020）. Conclusion：Nanog expression is relevant to the carcinogenesis and development of NSCLC,besides,the expression of Nanog protein is associated with the prognosis of patients with LAC.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2013年第6期758-761,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
广西壮族自治区卫生厅计划课题Z2011171
