摘要
目的:探讨孤束核在艾灸预处理保护胃黏膜机制中的作用。方法:健康SD大鼠40只随机分为4组,分别为:A正常对照组;B模型组;C艾灸+模型组;D艾灸+模型+孤束核损毁组,每组10只。运用脑立体定位及直流电损毁技术预先损毁大鼠双侧孤束核后进行足三里穴艾灸预处理,在无水乙醇灌胃法造模损伤大鼠胃黏膜后,进行大鼠胃黏膜损伤指数(UI)及病理切片观察,采用ELISA方法检测大鼠胃黏膜表皮生长因子(EGF)和生长抑素(SS)含量,并采用W-B方法检验HSP70在大鼠胃黏膜组织中的表达。结果:①B组胃黏膜出现明显损伤,表明采用无水乙醇灌胃胃黏膜损伤造模法造模成功。C、D组UI指数较B组均有不同程度的下降(P<0.01,P<0.05);D组UI指数较C组高(P<0.01)。②光镜观察结果显示A组胃黏膜组织内丰富的毛细血管,上皮结构完整,细胞排列有序;B组胃黏膜结构破坏,胃黏膜腺体细胞大量坏死,胃黏膜损伤明显;C组胃黏膜表面有部分脱落,细胞内充血情况明显改善;D组胃黏膜细胞内充血情况改善,但仍可见充血。③C、D组胃黏膜组织中EGF含量较B组均有不同程度的上升,SS含量均有下调(P<0.05,P<0.01);D组胃黏膜组织中EGF含量C组低,而SS含量较C组高(P<0.01)。④C、D组胃黏膜组织中HSP70表达较B组均有明显上升(P<0.01);D组较C组胃黏膜组织中HSP70表达低(P<0.05)。结论:艾灸预处理足三里穴对胃黏膜损伤有明显保护作用,而孤束核损毁对其保护效应有明显影响,证明孤束核是艾灸对胃黏膜损伤与保护因子表达与促进HSP70表达的重要调节部位,起到了重要的信号汇聚与整合的作用。
Objective： To discuss the effect of NTS on moxibustion pre-protection of the gastric mucosa. Methods： 40 SD rats were randomly divided into 4 groups： control group （Group A）, model group （Group B）, pre-moxibustion group （Group C）, and group of pre-moxibusfion＋damage nucleus of solitary tract （Group D）. Damaged bilateral NTS of Group D by brain stereotaxic and dc damaged technology, then applied moxibustion at Zusanli point （ST36）. After poured absolute ethanol into stomachs to injury the gastric mucosa of rats, to observe the UI and pathological section of gastric mucosa. The contents of EGF and SS in gastric mucosa were detected by ELISA, and the expressions of HSP70 in gastric mucosa were detected by Western-blot. Results： （~）After poured absolute ethanol into stomach, the gastric mucosa of Group B were injured obviously. It was illustrated that acute gastric mucosa injury model was made successfully. The UI of Group C and D was lower than that of Group B, more or less （P〈0.05, P〈0.01）, and the UI of Group C was lower than Group D （P〈0.01）. ~）The observation by optical microscope suggested that the gastric mucosa of Group A contained abundant capillaries, epithelial ceils arranged completely and orderly, the gastric mucosa of Group B was injured obviously and the structure of gastric mucosa was damaged, part of the cells fall off from the gastric mucosa of Group C and the congestion in cells had improved, the gastric mucosa of Group C still could find cells hyperaemia though it had improved. （~）The contents of EGF in gastric mucosa of Group C and D increased at different degrees and the contents of SS decreased than Group B （P〈0.05, P〈0.01）. The contents of EGF of Group D were lower than Group C while the contents of SS were higher （P〈0.01）. @The expression of HSP70 in gastric mucosa of Group C and D increased obviously than Group B （P〈0.01）, and Group D was lower than Group C （P〈0.05）. Conclusion： Moxibustion pretreatment at Zusanli point （ST36） has the pre- protection on acute gastric mucosa injury. After NTS damage, the gastric mucosa protective action of moxibustion pretreatment is weak. This proves that NTS had participate in signal transduction of the gastric mucosa protective action of moxibustion and can adjust the contents of protecting factors and the expressions of HSP70.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第12期3688-3693,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家973计划中医基础理论专项(No.2009CB522904)
国家自然科学基金项目(No.81173326
No.81303050)
湖南省科技计划项目(No.2011SK3095)
长沙市科技计划重点项目(No.K1005020-31)~~
