摘要
目的 在体外实验中 ,探索天花粉蛋白诱导胃癌细胞发生凋亡的可能性 ,揭示该凋亡发生与bcl 2之间的关系。方法 以细胞形态学和TUNEL染色法 ,定性、定量地研究天花粉蛋白与胃癌细胞凋亡的关系 ;通过免疫组化法和NorthernBlot杂交法检测凋亡相关基因bcl 2的表达。结果 天花粉蛋白在体外能诱导人胃癌细胞发生凋亡 ,下调bcl 2的表达。结论 诱导胃癌细胞凋亡是天花粉蛋白抗胃癌作用的机制之一 ,天花粉蛋白可能经下调凋亡相关基因bcl
Objective To investigate the apoptosis in gastric cancer induced by trichosanthin, and the relationship between this apoptosis and expression of bcl2. Methods In in vitro experiments, morphologic test and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of gastric adenocarcinoma cell line SGC7901 before and after the trichosanthin treatment. Immunohistochemical staining method and Northern Blot hybridization were used for detecting expression status of apoptosisrelated genes bcl2, before and after trichosanthin treatment. Results When SGC7901 cells were treated with trichosanthin (0.1 μg/ml, 36 h), they presented some typical apoptotic morphologic changes observed by fluorescent staining. These morphologic changes include nuclear condensation, nucleosomal fragments forming a lunate body under nuclear membrane, etc. When SGC7901 cells were treated with trichosanthin at the concentration 0.1 μg/ml for 36 h,42 h and 48 h, respectively, TUNEL staining showed a significant increase of apoptotic index (AI), from 3.78%±1.11%, 3.98%±1.12%,3.85%±1.08%, respectively, to 11.30% ± 2.33%, 10.22% ±2.00%,11.18%±1.85%(P<0.01). When trichosanthin was 0.1 μg/ml for 32 h, immunohistochemical staining showed a decreased expression of Bcl2 protein product, staining density decreased from ++～+++ to -～+ (P<0.01); when trichosanthin was 0.1 μg/ml for 24 h, Northern Blot hybridization showed a decreased expression of bcl2 RNA and hybridization decreased from 35.19±2.34 to 22.27 ± 3.90 (p< 0.01). Conclusions Trichosanthin is able to induce the apoptosis in gastric cancer. This apoptosis may be mediated by downexpression of apoptosisregulated gene bcl2.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2000年第6期380-382,共3页
Chinese Journal of Digestion
