摘要
研究内蒙古赤峰地区甜荞种质的遗传多样性,为种质资源合理利用提供依据。采用SSR分子标记对大部分来自赤峰地区的73份甜荞种质的遗传多样性进行了分析。15对SSR引物共扩增出77个条带,其中多态性条带有50条,占总数的64.9%。根据SSR扩增结果,应用NTSYSpc2.1数据分析软件计算各品种间的遗传相似系数表明,这些材料之间的遗传相似系数在0.78～0.96之间;在相似系数为0.81时,73份材料可分成4组,赤峰地区的58份材料在每一组中均有分布。赤峰地区的甜荞具有丰富的遗传多样性,具有很大的挖掘优良等位基因、进行选择育种的潜力,并发现了3份比较特异的材料。
To make good use of the germplasm resources for breeding,genetic diversity of common buckwheat landrace in Chifeng was analyzed. Using fifteen pairs of SSR primers,the genetic diversity and relationship was analyzed among seventy-three common buckwheat accessions. Seventy-seven bands were amplified,fifty of which were polymorphic,which accounted for 64.9%. Genetic coefficient of most accessions ranged from 0.78to 0.96 by software NTSYSpc2.1 based on SSR results. The seventy-three common buckwheat accessions could be divided into four groups on coefficient threshold of 0.81. The fifty-eight accessions from Chifeng were found in all groups. Common buckwheat accessions from Chifeng were of high biodiversity,so they had good potential to find excellent alleles and to carry out selective breeding. Meanwhile,three special materials were found.
出处
《中国农学通报》
CSCD
2013年第33期129-134,共6页
Chinese Agricultural Science Bulletin
基金
内蒙古自治区科技计划项目"荞麦新品种选育及配套栽培技术研究集成"(20110707)
赤峰市农牧科学研究院基金项目"赤峰地区荞麦种质资源遗传多样性研究及优良种质筛选"(201101)
关键词
甜荞
遗传多样性
SSR标记
赤峰
common buckwheat（Fagopyrum esculentum）
genetic diversity
SSR marker
Chifeng
