摘要
研究在培养基中加入硫胺素(V B1)对cAMP发酵的影响。结果表明:V B1的最适添加量为0.5 g/L,与对照组相比,环磷酸腺苷(cAMP)产量和细胞干质量分别提高了36.4%和41.8%,达到7.5和7.8 g/L。琥珀酸和α-酮戊二酸的含量有明显的提高,平均提高了43.59%和40.77%;同时,主要副产物乙酸的含量没有明显变化。在发酵过程中,V B1的加入对ATP的含量及与cAMP合成密切相关的几种酶的活性也有显著的影响。
The effects of addition of thiamine （VB1） on cyclic adenosine monophosphate （cAMP） production by Arthrobacter sp. A302 were investigated. The results showed that the addition of 0.5 g/L VB1 was optimum for cAMP production and cell growth. The cAMP yield and the biomass reached 7.5 g/L and 7.8 g/L, and they were improved by 36.4% and 41.8% , respectively, compared with the control. The concentrations of succinic acid and a-ketoglutarate increased by 43.59% and 40.77%, and the production of acetic acid had no obvious change by addition 0.5 g/L VB1. The addition of VB1 also affected the content of intracellular ATP and activities of several enzymes closely related to biosynthesis of cAMP.
出处
《生物加工过程》
CAS
CSCD
2013年第6期19-23,共5页
Chinese Journal of Bioprocess Engineering
基金
国家杰出青年基金(21025625)
国家高技术研究发展计划(863计划)(2012AA021203)
国家重点基础研究发展计划(973计划)(2011CBA00806)
国家自然科学基金青年基金(21106070)
教育部长江学者和创新发展计划(PCSIRT)
江苏省自然科学基金(SBK201150207)
江苏省高校优势学科建设工程项目(PAPD)
