摘要
[目的]观察昆布提取物（AEK）对小鼠免疫功能及抗疲劳能力的影响。[方法]昆明种小鼠,体质量18~22 g,40只,分为4组,分别为AEK小剂量组（21.2 g/kg）、AEK大剂量组（106 g/kg）、生理盐水空白对照组和阳性对照甲状腺素组（20 mg/kg）。灌胃给药10 d后,分别测定AEK对小鼠腹腔巨噬细胞吞噬功能和淋巴细胞转化功能的影响,以及对二硝基氯苯（DNCB）致耳肿胀和抗疲劳能力的影响。[结果]AEK可提高小鼠腹腔吞噬细胞的吞噬百分率及吞噬指数;促进植物凝血素（PHA）诱导的淋巴细胞转化;促进DNCB引起的皮肤迟发超敏反应及溶血素抗体的生成;延长小鼠在水中游泳时间。[结论]AEK对小鼠非特异性免疫、细胞免疫及体液免疫均有增强作用,并能提高小白鼠的抗疲劳能力。
[Objective] To observe the influences of aqueous extract of Kelp（AEK） on immune function and anti-fatigue in Mice.[Methods] Mice（18~22） g were divided into 4 groups： low dose group（AEK 21.2 g/kg）, high dose group（AEK 106 g/kg）, blank control group（ 0.9% NaCl）, positive control group（thyroxine 20 mg/kg）. Drugs were administered to mice by i.g. for 10 days. The phagocytic activity of peritoneal macrophages, transformation of lymphocyte, the production of serum haemolysis and skin delayed-type hypersensitivity reaction induced by DNCB were determined. Anti-fatigue effect was evaluated by the swimming time in water. [Results] AEK could improve phagocytic activity of peritoneal macrophages and transformation of lymphocyte, and promote mouse skin delayedtype hypersensitivity reaction induced by DNCB as well as increase production of the haemolysis. Swimming time was prolonged by AEK. [Conclusion] Non-specific, cellular and humoral immunity is promoted, and anti-fatigue effect is increased by AEK in mice.
出处
《天津中医药大学学报》
CAS
2013年第4期214-216,共3页
Journal of Tianjin University of Traditional Chinese Medicine
关键词
昆布
免疫功能
抗疲劳
小鼠
aqueous extract of Kelp
immune function
anti-fatigue effect
mice
