摘要
目的：建立一种快速测定苯巴比妥血药浓度的方法。方法：采用反相高效液相色谱法，以卡巴西平为内标，测定苯巴比妥血药浓度，钯谱柱ｓｈｉｍａｄｚｕ ｓｈｉｍｐａｃｋＣＬＣ－Ｃ１８不锈钢柱，流动相为甲醇－水（６０：４０），流速０．８ｍｌ／ｍｉｎ，检测波长２５４ｎｍ。结果：在５～４０ｕｇ／ｍｌ逍度范围内线性良好（ｒ＝０．９９９９），最低检测限为１１．５７ｎｇ／ｍｌ高、中、低三种浓度的平均回收率分别为１００．２４％，１００．２８％，９９．４１％，ＲＳＤ分别为０．７％，２．７％，５．８％（ｎ＝９）。日内和日间平均ＲＳＤ分别为３．３％，５．２％，８．７％和７．３％。７．３％，９．２％（ｎ＝９）。结论：该方法准确、快速、简便，灵敏度高，重现性好，可作为苯巴比妥血药浓度监测的常规方法。
ve: To establish a method for rapid determining phenobarbitone in human plasma. Methods: Using RP -HPLC with carbamazepine as standard, to determine concentration of phenobarbitone in human plasma. The separation wasperformed on shimadzu shimpack CLC - C18 column with methanol-water(60: 40)as mobile phase. The flow rate was 0. 8ml/min. The wavelength for measurement was 254nm. Results: The linear ranges of phenobarbitone was between 5 - 40 ug. ml-1 with r =0. 9999, The lowest limit of detection was 11. 57ng/ml, The average recoveries for phenobarbitone in three different concentration were 100. 24% , 100. 28% , 99. 41% , with RSD of 0. 7% , 2. 7% and 5. 89% respectively (n = 9). The RSD values of intraday and interday were 3. 3%, 5. 2% 8. 7% and 7. 3%, 7. 3%, 9. 2% (n=9). Conclution: This method is accurate, rapid and ease, with high sensitive and good repeatability. It can be used routine method by TDM.
出处
《儿科药学》
1999年第3期18-19,31,共3页
Journal of Pediatric Pharmacy
