摘要
目的:探讨P38丝裂原活化蛋白激酶(P38 mitogen-activated protein kinase,P38MAPK)信号通路在白细胞介素-1β(IL-1β)调节人牙周膜成纤维细胞中基质金属蛋白酶-1(MMP-1)表达中的作用。方法:体外分离培养的人牙周膜成纤维细胞(hPDLFs),随机将其分为:①对照组:加入等量无血清培养液;②实验组1:加入10 ng/mL IL-1β作用24 h;③实验组2:加入10μmol/L SB203580预处理30 min后,再加入10 ng/mL IL-1β作用24 h,然后分别采用噻唑蓝比色测定法(MTT)检测各组hPDLFs的增殖情况;Real-time PCR、免疫荧光技术及Western blot观察MMP-1各组mRNA和蛋白表达的变化。结果:各组MMP-1mRNA和蛋白的表达均以IL-1β作用组最高,IL-1β联合SB203580复合作用组次之,对照组最低,3组间两两比较,差异均具有统计学意义(P<0.05)。结论:IL-1β可通过激活p38MAPK途径促进人牙周膜成纤维细胞MMP-1的表达。
AIM: To investigate the effects of IL-113 on the expression of matrix metalloproteinase-1 ( MMP-1 ) in human periodontal ligament fibroblasts (hPDLFs) via P38 mitogenactivated protein kinase ( P38 MAPK) pathway. METHODS: hPDLFs were isolated and cuhuered in vitro, and then were randomly assigned to 3 groups. (1) In control group hPDLFs were cultured without any stimulus;(2) in IL-1β group hPI)LFs were incubated with 10 ng/mL of IL-1β for 24 h; (2) in IL-1β + SB203580 group hPDLFs were pretreated for 30 rain with the P38MAPK specific inhibitor SB203580 at 10 μmol/L, and then treated with 10 ng/mL of IL-1β for 24 h. The cell proliferation was examined by MTT assay, and MMP-1 expression was detected by RT-PCR, immuofluorenscence and Western blot. RESULTS : In the 3 groups cell proliferation activity was similar. The expression of MMP-1 mRNA and protein was the highest in the IL-1β group, moderate in IL-1β + SB203580 group and the lowest in control group (berween each 2groups, P 〈0.05). CONCLUSION: IL-1β can promote MMP-1 expression in human periodontal ligament fibroblasts via P38MAPK pathway.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2013年第12期748-752,共5页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(81070870)