摘要
本研究检测阻断Ras/Erk信号通路对原代人类急性淋巴细胞白血病(ALL)细胞重要转录因子c-fos、c-jun、TAK1基因表达的影响。筛选PD98059作用的最佳有效浓度和时间,采用SYBR GreenⅠ实时定量PCR检测PD98059作用于原代ALL细胞前后和正常人原代细胞的目的基因的表达水平。结果表明:与正常人原代细胞相比,处理前原代ALL细胞c-fos、TAK1 mRNA的相对表达升高(P值分别为0.014和0.017)。经PD98059处理后原代ALL细胞中c-fos、c-jun、TAK1 mRNA的相对表达情况为:c-fos、c-jun mRNA 7个样本均低表达,TAK1 mRNA 5个样本低表达,2个样本高表达;较处理前c-fos、c-jun、TAK1 mRNA表达明显下降(P值均为0.018)。处理后原代ALL细胞与正常人原代细胞相比,c-fos、c-jun、TAK1 mRNA表达无统计学差异。结论:原代ALL细胞的c-fos和TAK1基因的活性增高,阻断ALL细胞的Ras/Erk信号通路可导致重要转录因子c-fos、c-jun、TAK1基因表达明显下降。
This study was purposed to investigate the effect of blocking Ras/Erk signaling pathway on expression of important transcription factor c-fos, c-jun and TAK1 gene in primaliry acute lymphocytic leukemia （ALL） cells. The best effective concentration and effect time of PD98059 were screened; the expression levels of c-fos, c-jun and TAKI in primaliry cultured cells of normal persons, primality cultured ALL ceils and primaliry cultured ALL ceils treated by PD98059 were detected by SYBR Green I real-time quantitative-PCR. The results showed that before trantment by PD98059 the expression levels of c-fos and TAK1 mRNA were significantly up-regulated in primary cultured ALL cells as compared with primaliry cultured cells of normal presons （P = 0.014 and P = 0. 017 respectively）. After treatment by PD98059, the expression levels of c-fos, c-jun mRNA decreased in all 7 serum samples, while expression of TAK1 was down-regulated in 5 samples, and up-regulated in 2 samples. After treatment with PD98059, there was no statistical difference of c-fos, c-jun and TAK1 expression levels in primaliry cultured ALL ceils and primaliry cultured normal cells. It is concluded that the c-fos and TAK1 activity of primaliry cultured ALL cells increases, and blocking the Ras/Erk signaling pathway of ALL cells can lead to obvious decrease of important transcription factors c-fos, c-jun, TAK1 genes expression.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2013年第6期1399-1402,共4页
Journal of Experimental Hematology
基金
国家自然科学基金青年科学基金(编号81000921)
