摘要
利用分子克隆技术成功获得麦洼牦牛HOXA6基因编码区序列,并结合内参基因通过实时相对定量PCR法检测HOXA6基因在麦洼牦牛各组织中的表达分布。采用生物信息学方法对该基因及其编码的蛋白进行基本理化性质、疏水性、二级结构等分析,并构建原核重组表达系统。结果表明,该基因包含一个大小为702 bp的开放阅读框,编码233个氨基酸;所编码的蛋白属于亲水性蛋白,二级结构主要由无规则卷曲和α螺旋为主。HOXA6基因的编码产物氨基酸进化树表明,HOXA6基因进化极其保守,牦牛HOXA6与牛、绵羊、马等物种氨基酸遗传距离较近。对其编码区序列比对显示,该序列具有高度保守性,与其他物种同源性较高。
A coding region sequence of Maiwa yak（Bos grunnien）HOXA6 was cloned in this research.Tissue expression patterns were detected by RT-PCR in combination with the reference gene（GAPDH ）. Some characters of the HOXA6 gene and encoded protein sequences were analyzed by the methods of bioinformatics in the following aspects as the general physical and chemical properties, hydrophobicityand secondary structure. Prokaryotic expression system was also constructed in the present study. Results showed that the coding region sequence of Maiwa yak HOXA6 contains a complete ORF（702 bp）which encode 233 amino acids. The protein encoded by Maiwa yak HOXA6 is a hydrophilic protein, and the secondary structures are mainly composed of a-helix and random coli. The phylogenetic tree shows that the protein of Maiwa yak was so closed to cattle, sheep and horse. Sequence alignment of HOXA6 gene showed highly homology in yak with other species.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第12期88-93,共6页
Biotechnology Bulletin
基金
国家科技支撑计划(2012BAD13B06)
西南民族大学中央高校基本科研业务费专项资金(13NZYQN24
TS11126)
