摘要
野生大豆是栽培大豆的原始祖先种,其遗传多样性远远超过栽培大豆,并且蕴藏着多种优异抗性基因。目前关于野生大豆优异基因挖掘和利用的研究很少,本研究概述了转录组测序的基本原理、实验流程、数据分析和应用现状,并展望了将转录组测序技术应用于野生大豆的研究,可能对野生大豆资源基因发掘和利用及其在大豆种质创新与品种设计育种中产生的影响。将野生大豆研究深入到分子水平,对野生大豆资源的深入研究具有重要理论意义和实践价值。
As the original ancestor of the cultivated soybean, wild soybean has abundant genetic diversity and holds a variety of excellent genes. The molecular and genomic research of the wild soybean genes was less documented. This study summarized the basic principle, experiment process, data analysis and the current application status of transcription sequencing, and also prospected the influence on the gene resources exploitation and germplasm enhancement and molecular design breeding of soybean based on the application of transcription sequencing to wild soybean. The in-depth research of wild soybean to the molecular level has great theoretical significance and practical value to excavation and utilization of the wild soybean resources.
出处
《大豆科学》
CAS
CSCD
北大核心
2013年第6期845-851,共7页
Soybean Science
基金
国家高技术研究发展计划"863计划"(2011AA10A105)
现代农业产业技术体系建设专项(CARS-04-PS07)
关键词
野生大豆
基因资源
转录组测序
挖掘和利用
Wild soybean
Gene resource
Transcription sequencing
Excavate and utilize
