摘要
采用氯磺酸—吡啶法和浓硫酸法制备硫酸化小刺猴头菌多糖,傅立叶变换红外光谱鉴定硫酸化多糖。结果表明:修饰后的多糖在1 259.17 cm-1和807.73 cm-1或1 226.44 cm-1处出现硫酸酯键吸收峰,表明硫酸基团已经与多糖结合成酯。采用氯化钡—明胶法测定硫酸根含量,制备取代度为0.94或0.82的硫酸化多糖。
Sulfated Hericium caput-medusae polysaccharides were prepared through wolfrom and sulfuric acid methods and the obtained polysaccharides were characterized by Fourier-transform infrared spectra. The results showed the polysaceharide emerged absorption peak corresponded to sulfate ester at 1 259.17 cm^-1 and 807.73cm^-1 or 1 226.44 cm^-1 after modification, indicating the group of sulphuric acid had conjugated ester with polysaccharide. The content of sulphate was determined through barium- chloride gelatin procedures. Sulfated polysaecharides with substitution degrees of 0.94 and 0.82 were prepared.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2013年第6期704-707,共4页
Journal of Jilin Agricultural University
基金
国家科技支撑计划项目(2007BAI38B06-03)
关键词
小刺猴头菌
多糖
氯磺酸-吡啶法
浓硫酸法
氯化钡-明胶法
Hericium caput-medusae
polysaccharide
wolfrom method
sulfuric acid method
barium-chloride gelatin
