摘要
建立了利用固相萃取一高效液相色谱法同时测定肉制品中4种合成色素的检测方法。样品经乙醇、氨水和水溶液（7：2：1，m/m）提取后，固相萃取净化，经C18柱分离，用紫外检测器检测。结果表明：4种合成色素分离良好，加标平均回收率为66．7％～99．7％，相对标准偏差1．9％～3．6％。该方法准确度和精密度较高，可应用于测定肉制品中的合成色素。
This paper studied a method for simultaneous determination of 4 synthetic food colorants including amaranth, carmine, sunset yellow and allure red in meat products by reversed-phase HPLC following solid phase extraction. Samples were extracted with ethanol-aqueous ammonia-water （7：2：1, m/m） and after clean-up by solid phase extraction, the extracts were separated on a C18 cartridge and detected with a UV detector. Good separation was achieved for the four food colorants. The mean spiked recoveries were in the range from 66.7% to 99.7% with RSD between 1.9% and 3.6%. The HPLC method was highly accurate and precise and could be used for the determination of food colorants in meat products.
出处
《肉类研究》
2013年第10期26-28,共3页
Meat Research
关键词
合成色素
固相萃取
反相高效液相色谱法
synthetic colorant
solid phase extraction
RP-HPLC
meat products
