摘要
目的探讨硫酸镁对急性肺栓塞肺动脉高压的影响及其抗炎机制。方法 18只杂种犬,随机分为空白组、对照组及硫酸镁2.0 mm/kg组(MgSO42.0组),每组6只。其中空白组不给于任何处理,对照组只给予处理造成急性肺动脉高压,MgSO42.0组造成急性肺动脉高压稳定30 min后,以2.0 mm/kg10 min内静脉滴注给予硫酸镁;肺动脉、股动脉插管检测平均肺动脉压(MPAP)、体循环平均动脉压(MAP),应用酶联免疫吸附以及放免法分别测定血清中白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α),western blot测定肺组织中MCP-1蛋白表达。结果对照组MPAP较空白组显著升高,MgSO42.0组MPAP较对照组显著下降(P<0.05或<0.01);对照组MAP较空白组显著下降,MgSO42.0组MAP较对照组显著升高(P<0.05或<0.01);对照组血清中TNF-α以及IL-6空白组显著升高,MgSO42.0组TNF-α以及IL-6较对照组显著下降(P<0.05或<0.01);对照组MCP-1蛋白表达较空白组显著升高,MgSO42.0组MCP-1蛋白表达较对照组显著下降(P<0.05或<0.01)。结论硫酸镁可显著降低肺动脉高压,这可能与其抑制肺组织MCP-1表达,降低TNF-α以及IL-6,从而抑制炎性反应增强有关。
Objective To investigate the effect of magnesium sulfate on acute pulmonary hypertension and its anti-inflammatory mechanism in mongrel dogs. Methods 18 mongrel dogs were randomly divided into three groups：blank group, control group and magnesium sulfate group （2.0mm/kg） , with 6 dogs in each group. The dogs in blank group were not given any treatment, the dogs in control group were operated to induce acute pulmonary hypertension,and the dogs in magnesium sulfate group were given magnesium sulfate 2.0mm/kg by intravenous infusion for 10 minutes after the animal models with acute pulmonary hypertension were established. The mean pulmonary artery pressure （MPAP） and circulation mean arterial pressure （MAP） were detected by pulmonary artery and femoral artery cannulation, IL-6 and TNF--α levels in serum were detected by enzyme-linked immunosorbent assay （ELISA） and radioimmunoassay, respectively, and MCP-1 protein expression was determined by Western blot. Results The MPAP in control group was significantly higher than that in blank group; however MPAP in magnesium sulfate group was significantly lower than that in control group （ P 〈 0.05 or P 〈 0. O1 ）. The MAP in control group was significantly lower than that in blank group, however, MAP in magnesium sulfate group was significantly higher than that in control group （ P 〈0.05 orP 〈0.01 ）. The serum levels of TNF-α and IL-6 in control group were significantly higher that those in blank group, however, which in magnesium sulfate group were significantly lower than those in control group （ P 〈 0.05 or P 〈 0.01 ）. The MCP-1 protein expression levels in control group were significantly higher than those in blank group, however, which in magnesium sulfate group was significantly lower than those in control group （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Magnesium sulfate can significantly reduce acute pulmonary hypertension ,which may be related to its effects of inhibiting the expression of MCP-1 in lung tissue, decrease TNF-α and IL-6 levels so as to inhibit inflammatory response.
出处
《河北医药》
CAS
2013年第23期3540-3542,共3页
Hebei Medical Journal
关键词
杂种犬
急性肺动脉高压
炎性反应
MCP-1
mongrel dogs
acute pulmonary hypertension
inflammatory response
MCP-1
