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脂肽-糖脂混合生物表面活性剂产生菌筛选和优化培养 被引量:12

Identification of Bacillus subtilis THY-7 and high titer optimization for the blend-biosurfactant of lipopeptide and glycolipid
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摘要 结合脂肽和糖脂的性能优势,致力于产脂肽-鼠李糖脂混合型生物表面活性剂的新菌株选育和培养条件优化。采用血平板溶血圈法初筛菌株、改进排油圈法快速检测产量以及飞行时间质谱鉴定产物结构。对优选菌株的碳源、氮源和磷酸盐缓冲液、重要金属离子浓度等进行了单因子和正交试验,优化了培养基和培养条件。采用高压液相色谱和蒽酮比色法定量分析了产物组成。筛选获得了同时积累糖脂和脂肽的新菌株,鉴定命名为芽胞杆菌Bacillus subtilis THY-7。摇瓶分批培养48 h,细胞OD600为37.0,产物浓度2.4 g/L,分别是优化前的3.4倍和3.1倍。发酵罐补料分批培养,泡沫中产物浓度达到4.5 g/L,且74%为表面活性素,22%为鼠李糖脂。B.subtilis THY-7是具有脂肽-鼠李糖脂高产潜力的优选菌株。 Biosurfactants (BSs) are highlighted owing to their multiple advantages in diverse applications. To screen a superior strain that producing a blend-biosurfactant of lipopeptide and glycolipid, the hemolytic activity assay on blood agar plates, the modified oil-red spreading test and MALDI-TOF Mass Spectrometry identification of the purified products was carried out. Bacillus subtilis THY-7 was selected and its principal products were surfactin and dirhamnolipid. The medium component and culture conditions of THY-7 were optimized by both single factor and orthogonal experiments. After 48 h optimal batch culture in flask, the cell density (OO600) was 37.0 and the product titer was 2.4 g/L, which was 3.4 folds and 3.1 folds of that under original condition, respectively. A fed-batch culture in a 5 L fermentor was further performed coupling with in situ recovery of foam, in which the titer of blend-BS increased to 4.5 g/L at 25 h. Quantification by HPLC and anthrone colorimetry revealed that surfactin and dirhamnolipid accounted for 74% and 22% of the blend-BS, respectively.
出处 《生物工程学报》 CAS CSCD 北大核心 2013年第12期1870-1874,共5页 Chinese Journal of Biotechnology
基金 国家重点基础研究发展计划(973计划)(No.2013CB733600) 清华大学自主科研计划(No.20111081120)资助~~
关键词 脂肽和糖脂 混合型生物表面活性剂 枯草芽胞杆菌 质谱鉴定 正交优化 定量分析 lipopeptide and glycolipid, blend-biosurfactant, Bacillus subtilis, mass spectrometry identification, orthogonaloptimization, quantification analysis
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参考文献14

  • 1Sen R. Biosurfactants. Austin: Landes Bioscience/Springer Science+Business Media, LLC, 2010:316-322.
  • 2Banat IM, Makkar RS, Cameotra SS. Potential applications of microbial surfactants. Appl Microbiol Biotechnol, 2000, 53(5): 495-508.
  • 3Jarvis FG, Johnson MJ. A glycolipid produced by Pseudomonas aeruginosa. J Am Chem Soc, 1949, 71(12): 4124-4126.
  • 4Wang QH, Fang XD, Bai B J, et al. Engineering bacteria for production of rhamnolipid as an agent for enhanced oil recovery. Biotechnol Bioeng, 2007, 98(4): 842 853.
  • 5Nie M, Yin X, Ren C, et al. Novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium Pseudomonas aeruginosa strain NY3. Biotechnol Adv, 2010, 28(5): 635-643.
  • 6Onwosi CO, Odibo FJC. Effects of carbon and nitrogen sources on rhamnolipid biosurfactant production by Pseudomonas nitroreducens isolated from soil. World J Microbiol Biotechnol, 2012, 28(3): 937-942.
  • 7Kim PI, Ryu J, Kim YH, et al. Production of biosurfactant lipopeptides iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J Microbiol Biotechnol, 2010, 20(1): 138-145.
  • 8Seydlovt G, Svobodovfi J. Review of surfactin chemical properties and the potential biomedical applications. Cent Eur J Med, 2008, 3(2): 123-133.
  • 9Iglauer S, Wu Y, Shuler P, et al. New surfactant classes for enhanced oil recovery and their tertiary oil recovery potential. J Pet Sci Eng, 2010, 71(1): 23-29.
  • 10Janek T, Lukaszewiez M, Rezanka T, et al. Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard. Bioresour Technol, 2010, 101(15): 6118-6123.

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