摘要
目的探讨CXCLl2／CXCR4生物轴对胰腺癌细胞增殖、侵袭等生物学行为的影响。方法体外培养胰腺癌细胞系Miapaca-2，将其分为对照组、CXCLl2组和AMD3100组。（1）采用RT—PCR检测胰腺癌细胞中CXCLl2、CXCR4、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）和人尿激酶型纤溶酶原激活物（uPA）mRNA的表达水平；（2）采用CCK-8法检测各组细胞的增殖情况；（3）采用Transwell侵袭实验检测CXCLl2／CXCR4对胰腺癌细胞趋化活性的影响。结果胰腺癌细胞系Miapaca-2中CXCLl2mRNA未见表达，而CXCR4mRNA在胰腺癌细胞中有表达。MMP-2、MMPO和uPAmRNA在AMD3100组、对照组和CXCLl2组中的表达水平呈递增趋势，差异具有统计学意义（P〈0．05）。胰腺癌细胞的增殖和侵袭能力在CXCLl2组明显增强，而在AMD3100组得到了有效的抑制，组间差异有统计学意义（P〈0．05）。结论趋化因子CXCLl2及其受体CXCR4所构成的生物轴对胰腺癌细胞的增殖和侵袭能力发挥着重要的作用。
Objective The aim of this study is to explore the effect of CXCL12/CXCR4 biological axis on biological behavior of pancreatic cancer cells through the detection of mRNA expressions. Methods Pancreatic cancer cells （Miapaca-2） were cultured in vitro and divided into the control group, CXCL12 group and AMD3100 group. （1）RT-PCR was applied to detect the mRNA expressions of CXCL12, CXCR4, matrix metalloproteinase 2 （MMP-2） , MMP-9 and human urokinase plasminogen activator （uPA） ; （2） CCK-8 methods was used to detect the proliferation of cells; （ 3 ） Transwell invasion assay was applied to detect the invasive ability of pancreatic cancer cells among groups respectively. Results The mRNA expression of CXCL12 was not found, while the mRNA expression of CXCR4 was observed in pancreatic cancer cells. There was a tendency of decreasing on the mRNA expressions of MMP-2, MMP-9 and uPA among three groups, and there was statistically significant difference （ P 〈 0. 05 ）. The proliferative and invasive ability of pancreatic cancer cells showed an enhanced trend in CXCL12 group, while in AMD3100 group was effectively inhibited. There was statistically significant difference among three groups （ P 〈 0.05 ）. Conclusions CXCL12/CXCR4 biological axis plays an important role in proliferation and invasion of pancreatic cancer cells.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2013年第6期432-434,共3页
Chinese Journal of Experimental and Clinical Virology
