摘要
目的建立不同平面脊髓损伤所致神经源性膀胱大鼠模型，研究脊休克期后尿动力学及逼尿肌P2X1受体表达的变化。方法40只成年雌性Wistar大鼠分为对照组、骶上脊髓（胸腰段）损伤组、骶髓损伤组。骶上脊髓（胸腰段）损伤组于脊髓T10-L2节段横断脊髓，骶髓损伤组于脊髓T10-L2节段横断脊髓。术后6周，对3组大鼠行尿动力学检测，应用免疫组织化学方法检测P2X1受体在对照组大鼠、骶上脊髓（胸腰段）损伤组大鼠、骶髓损伤组大鼠逼尿肌中的表达。结果尿动力学检测骶上脊髓（胸腰段）损伤组大鼠膀胱充盈期逼尿肌反射亢进，逼尿肌漏尿点压力升高，膀胱顺应性增加；骶髓损伤组大鼠膀胱充盈期逼尿肌反射低下，逼尿肌漏尿点压力降低，膀胱顺应性增加。骶上脊髓（胸腰段）损伤组大鼠逼尿肌P2X1受体表达水平增高，骶髓损伤组大鼠逼尿肌P2X1受体表达水平降低。结论不同平面脊髓损伤致神经源性膀胱脊休克期后尿动力学发生明显改变，逼尿肌P2X1受体表达增强可能是逼尿肌反射亢进机制之一。
Objective To build the rat animal model of neurogenic bladder induced by spinal cord injury and investigate the changes of urodynamics and P2X1 receptor expression in bladder detrusor after the spinal shock stage. Methods A total of 40 female Wistar rats were randomly allocated into control group, suprasacral injury group and sa- cral injury group. Complete spinal cord transection was established by cutting out of dura at Tl0 - L2 in suprasacral inju- ry group. The sacral injury group was done by cutting out of dura at S2 -4. Urodynamic monitor was performed on rats af- ter the spinal shock stage （6 weeks after operation）. The P2X1 receptor expression in bladder detrusor among the 3 groups was checked by using immunohistochemical method. Results The suprasacral injury group exhibited detrusor hyperreflexia during bladder filling, elevated leak point pressure and compliance. The sacral injury group exhibited de- trusor lower reflection,reduced leak point pressure and elevated compliance. The level of P2X1 receptor expression in rats＇ detrusor of suprasacral injury group was increased, and the level of sacral injury group was reduced. Conclusions Neurogenic bladder induced by spinal cord injury reveals significant changes in urodynamic after the spinal shock stage. The enhancement of the P2X1 receptor expression in detrusor may be one of the mechanisms of detrusor hyperre- flexia.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第23期1784-1786,共3页
Chinese Journal of Applied Clinical Pediatrics
基金
北京市教育委员会科技计划面上项目（KM0111002-5020）
