摘要
目的:评价左旋聚乳酸/壳聚糖纳米纤维/纳米羟基磷灰石(poly(L-latic acid)/chitosan nano-fibers/nano-hydroxylapatite,PLLA/CSNF/nHA)复合仿生支架联合骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)修复新西兰兔桡骨大段缺损的可行性。方法:二次相分离法制作PLLA/CSNF/nHA仿生支架,并与新西兰兔的3代BMSCs复合。制作新西兰兔桡骨1.5 cm段状缺损模型27只,实验组缺损处植入PLLA/CSNF/nHA/BMSCs复合物、对照组植入PLLA/CSNF/nHA仿生支架、空白组不做处理,于术后第4、10、16周通过大体形态观察、放射学检查和组织学检查等方法评价缺损修复情况。结果:术后末次观察,实验组已完成缺损修复,材料完全降解;对照组部分修复,仍残留部分缺损;空白组缺损主要由纤维结缔组织填充。结论:PLLA/CSNF/nHA复合支架的仿生结构具有良好的生物相容性,联合BMSCs在生物体内可以加快新骨生成,完成新西兰兔大段骨缺损的修复。
Aim：To evaluate the feasibility of the biomimetic poly （L-latic acid ）/chitosan nano-fi-bers/nano-hydroxylapatite （PLLA/CSNF/nHA）composite scaffolds combined with bone marrow mesen-chymal stem cells （BMSCs）to repair bone defect of New Zealand rabbits.Methods：PLLA/CSNF/nHA composite scaffolds were prepared through the dual phase separation technique,then co-cultured with BM-SCs.The large defects （1.5 cm）in radius of rabbits were prepared and experimented in three groups. PLLA/CSNF/nHA/BMSCs compound was planted into the defected position in the experimental group;PLLA/CSNF/nHA scaffolds in the control;and no implant in the blank.The curative effect was evalua-ted by general -observation,radiology examination and HE staining at the 4th,1 0th and 1 6th week.Re-sults：The defects were repaired perfectly with the scaffolds degraded in the experimental group;partially repaired and degraded in the control;and full of fibrillar connective tissues in the blank,by the last eval-uation.Conclusion：PLLA/CSNF/nHA biomimetic scaffolds composited with BMSCs of rabbits have a good role to accelerate the formation of new bone and repair the large bone defect.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2013年第6期593-598,共6页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省科技计划项目(2011B050300013)
澳门科学技术发展基金项目(025/2010/A)
广东省中医药管理局基金项目(20111174)
关键词
骨髓间充质干细胞
骨缺损
壳聚糖
左旋聚乳酸
仿生支架
骨组织工程
bone marrow mesenchymal stem cells
bone defect
chitosan
poly （L-latic acid）
biomimetic scaffold
bone tissue engineering
