摘要
以狼尾蕨无菌苗叶片为外植体,探讨不同浓度的氯化钠(NaCl)对叶片诱导不定芽的影响。直接筛选法结果显示:当NaCl浓度为0(对照)时,叶片不定芽诱导率为80.2%;0.4%、0.5%、0.6%和0.7%NaCl处理下,不定芽诱导率分别降至28.4%、20.6%、15.9%和7.8%;0.8%NaCl处理下,叶片完全丧失不定芽的诱导能力。SSR分子标记检测结果显示,248对引物组合中有9对引物在狼尾蕨野生型和耐盐筛选植株间PCR扩增存在多态,其中引物WXE-56和WXE-204可作为耐盐突变体SSR分子标记筛选的适宜引物。
The leaves of sterile plantlet of Davallia bullata were used as materials to study the effect of different con- centrations of sodium chloride （NaC1） on induction of the adventitious bud. The induced rate of adventitious bud without NaC1 treatment（CK） was 80. 2%, and the induced rate treated by 0.4%, 0. 5%, 0. 6%, 0. 7% and 0. 8% NaC1 droped to 28.4%, 20. 6%, 15.9%, 7.8% and 0, respectively. Among 248 pairs of specific simple sequence repeat（SSR） prim- ers, nine pairs were associated with the salt tolerance of somatic mutants, among which, WXE-56 and WXE-204, were suitable for identification of the salt-tolerant mutant.
出处
《江苏农业学报》
CSCD
北大核心
2013年第6期1266-1270,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省科技厅科技支撑项目(BE2010339)
江苏省农业科技自主创新基金项目[CX(13)2044]
关键词
狼尾蕨
叶片
不定芽
体细胞突变体
耐盐
SSR标记
Davallia bullata.
leaf
adventitious bud
somatic mutant
salt tolerance
simple sequence repeat （SSR） marker
