摘要
目的：建立同时测定金粟兰属植物中香豆素类成分异嗪吡啶及倍半萜内酯类成分长尾粗木叶内酯和党参内酯3种成分含量的高效液相色谱方法。方法：采用Hypersil C18柱（4．6mm×250mm，5μm），乙腈-0．1％磷酸水溶液为流动相梯度洗脱，柱温30℃，检测波长230nm，流速1．0mL·min^-1。结果：异嗪吡啶、长尾粗木叶内酯、党参内酯线性范围分别为2～100，2～100，4～200ng，在上述范围内3种成分峰面积与进样量呈良好线性关系，相关系数r均〉0．999，上述3种成分的加样回收率分别为96．1％（RSD2．3％），94．4％（RSD2．0％），94．2％（RSD1．9％）；异嗪吡啶在多穗金粟兰中含量较高，最高含量为18μg·g^-1，长尾粗木叶内酯和党参内酯在丝穗金粟兰中含量较高，分别为2．4，1．2μg.g^-1结论：该方法简便快速，分离效果好，重复性好，可为金粟兰属植物的质量控制提供重要参考。
Objective： To develop an HPLC method for the simultaneous determination of three constituents about isofraxidin, lasianthuslactone and codonolactone. Method： The separation was performed on a Hypersil Cls column （4.6 mm ×250 mm, 5 μm） eluted with acetonitle and 0. 1% phosphoric acid solution as mobile phase in a linear gradient mode. The flow rate was kept at 1 mL .min-land the column temperature was set to 30 ~C , the UV detector wavelength was set at 230 nm. Result： The linear ranges were 2-100 ng for isofraxidin, 2-100 ng for lasianthuslactone, 4-200 ng for codonolactone, and the three components had a good linear relationship （r 〉0. 999）. Result of three components recovery tests were 96. 1% （ RSD 2.3% ）, 94.4% （RSD 2.0% ） , 94.2% （RSD 1.9% ）. The content of isofraxidin is more in Chloranhus multistachy, it is 18 p,g .g-I ; l the codonolactone and lasianthuslactone were higher in C. fortumei, the content was 2.4, 1.2 p,g .g Conclusion： Through the validation, the method is proved to be sensitive, accurate, repeatable, and can be used for quality control of C. plants.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第1期47-50,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(20962012
81260690)
江西省科技支撑计划项目(20111BBG70004-2)
江西省教育厅科学技术研究重点项目(GJJ13618
GJJ12518)
江西省高校中青年教师发展计划访问学者项目
