摘要
目的通过检测COPD患者外周血中Th17细胞、CD4。Treg细胞占CD4＋T细胞的比例及其比值、血浆中的弹性蛋白抗体水平，分析它们在c（）PD发病中可能的作用。方法采用流式细胞分析技术检测所有受试者外周血Thl7细胞及CD4＋Treg细胞占CD4＋T细胞的比例，酶联免疫吸附法检测血浆中弹性蛋白抗体水平。结果①c（）PD患者急性加重期组外周血Thl7细胞占CD4＋T细胞的比例，显著高于缓解期组和健康对照组，缓解期组显著高于健康非吸烟组，差异有统计学意义（P〈20．05），缓解期组高于健康吸烟组，但两者相比较差异无统计学意义。与健康非吸烟组相比，COPD急性加重期组、缓解期组和健康吸烟组外周血CD4。Treg细胞占CD4＋T细胞的比例显著增高，差异有统计学意义（Pt〈o．05），缓解期略高于急性加重期，高于健康吸烟对照组，但三者比较差异无统计学意义。急性加重期Thl7／CD4＋Treg的比值明显增高，与缓解期组、健康吸烟组和健康非吸烟组相比差异有统订学意义（P〈20．05）。②各组均检测到弹性蛋白抗体，COPD组、健康吸烟组的弹性蛋白抗体水平较健康非吸烟组增高，差异有统计学意义（P〈O．05），但急性期组、缓解期组、健康吸烟组之间差异无统计学意义。③c（）PD急性加重期组、缓解期组Thl7与弹性蛋白抗体呈正相关（P〈20．05）。COPD缓解期组Th17与FEV．％pred和FEV，／FVC呈显著负相关（P〈O．08）。结论Thl7细胞、CD4＋Treg细胞及弹性蛋白抗体可能参与了COPD的发生和发展。Thl7细胞增高、CD4＋Treg细胞增高相对不足与COPD发病及发展密切相关。Thl7在COPD中的表达增高可能加重了肺组织的破坏，这提示它可能在COPD肺气肿弹性蛋白免疫学特异性表达中发挥重要作用。
Objective To investigate the proportion of blood Thl7 and CD4＋ regulatory T cells （Treg） and plasma elastin antibodies quantity in patients with chronic obstructive pulmonary disease （COPD）, and to analysis their possible role in the development of COPD. Methods Th17 and CD4＋ Treg were quantified by flow cytometry in peripheral blood of all subjects, ELISA was used to determine the levels of elastin antibodies in their plasma. Results （1）The percentage of Thl7 on peripheral blood CD4＋ T in COPD acute exacerbation was significantly higher than catabasis and normal control group, and catabasis was significantly higher than normal no smoking control group （ P 〈 0.05 ）, no significant difference between COPD catabasis and normal smoking control group. The percentage of CD4 ＋ Treg on peripheral blood CD4＋ T in COPD acute exacerbation and catabasis and normal smoking control group was significantly higher than normal no-smoking control group （ P 〈0.05） , but no significant difference was found compared the three groups. Thl7 and CD4＋ Treg ratio in COPD acute exacerbation wassignificantly higher than catabasis and normal control group （P 〈2 0.05）. （2） Elastin antibodies was detected in all subjects, the content of elastin antibodies in plasma of normal no-smoking control group was significantly lower than COPD acute exacerbation and catabasis and normal smoking control group （P〈70.05）, but compared COPD acute exacerbation and catabasis and normal smoking control group, no significant difference was found. （3）A positive correlation between Thl 7 cells and Elastin antibodies was found in COPD acute exacerbation and catabasis. Thl7 arid FEV1 % pred and FEV1/FVC pr（sented negative correlation in COPD catabasis. Conclusions be implicated in the pathogenesis of COPD. Thl7 shortage are closely associated with development of COPD. Increase of Thl7 expression maybe increase the destruction of lung tissue, suggesting that it maybe play an important role in the COPD pulmonary emphysema elastic protein immunology specific expression.
出处
《国际呼吸杂志》
2014年第1期31-35,共5页
International Journal of Respiration
基金
2009年山西省留学人员科技活动项目择优资助项目（晋人字[2005]81号）
2010年度留学人员科技活动项目择优资助项目（人社厅函[2010]412号）
