摘要
目的:观察1.5T MRI检出大鼠 性脑损伤的能力。材料和方法:Wistar大鼠30只,毛果云香碱350mg/kg腹腔注射,诱发反复强直-痉挛全面大发作持续状态,HE染色,光镜下观察癫 持续不同时间脑组织损害程度,并与 1.5T MR成像对比(MRI参数: SE序列 T1加权像:重复时间=500ms,回波时间= 15ms; FSE序列 T2加权像:重复时间=400ms,回波时间=100ms;用表面线圈,层厚2.2mm,无间隔)。结果:癫 持续时间与脑损伤呈相关性改变。癫 持续 3h后,可见颞叶、海马区严重神经元脱失、胶质增生和脑水肿; MR扫描除在1只发作持续18h的大鼠左颞区检出长T1、长T2异常信号外(光镜提示为出血灶),未发现其余动物脑的结构异常和信号变化。结论:随着动物大发作持续时间延长, 性脑损害加重,大鼠癫 持续3h以上,有明显的海马神经元坏死及胶质增生,形成典型的海马硬化;1.5T MR常规序列扫描尚不能检出癫 大鼠早期脑改变。
Purpose: To observe the ability of demonstration of epilepsy caused brain injury with MRI in epilepto- genie rats. Materials and Methods: Thirty Wistar rats were injected with 10% pilocarpine intraperi- toneally to induce a generalized tonic - clonic seizure(GTCS). The degrees of brain injury with different duration of epilepsy were observed with light microscope and compared with 1. 5 T MRI findings(SE: T1 WI , FSE: T2 WI, Coil: surf are coil , Thickness: 2. 2mm without gap). Results: The duration of GTCS and the degree of brain injury in pathological study correlated fairly. After3 hours continuous GTCS, severe neuronal loss , ghosts and edema in the temporal lobe and hippocampal region were found by Pathology ,and no abnormality was revealed with MRI, MRI showed low signal intensity on T1 WI and high signal intensity on T2WI images in the left temporal lobe in one rat with GTCS lasting 18 hours. These abnormal signals were from hemorrhage confirmed by pathology.None of the remained rats was found abnormality on MRI. Conclusion: GTCS lasting for 3 hours can induce apparent neuronal less and ghosts, which means the formation mesial temporal sclerosis. The neuronal loss, gliosis and edema of brain in small animals such as rat cannot be demonstrated with conventional MRI sequences in acute epileptic animal models.
出处
《中国医学计算机成像杂志》
CSCD
2000年第5期344-346,共3页
Chinese Computed Medical Imaging