摘要
目的研究高效液相色谱法和胶乳凝集法测定糖化血红蛋白的相关性及胶乳凝集法测定时，离心力及取样量对检验结果的影响。方法同一组标本分别用高效液相色谱法和胶乳凝集法进行HbAlC％检测，分析两种检测结果的相关性及某些因素对检测的影响。结果两种方法检测HbAlC％结果具有较好的一致性；10μL红细胞＋1000μL溶血剂与20μL红细胞＋2000μL溶血剂对HbA1c％的测定结果差异无统计学意义（P〉0．05），选择4000r／min离心5min最适合的离心时间。结论使用离心标本检测HbA1c％的方法影响因素相对较多，在标本离心，吸取标本等环节均需严格控制，选择合适的取血量和离心时间。
Objective Studies the relevance of HPLC and Latex agglutination method in examing glycated he moglob and the influence of centrifugatl force and specimen volume to latex agglutination method. Methods The HbA1c% of the identical group specimen with HPLC and Latex agglutination assay , the relvance between the two methods and influencing of certain factors was examed. Results The results of two methods of exam in HbAlcY00 have good consistency;the level of different concentrations of which 10ul red blood cells added into 1 000 μL hemolytic agent or 20μL red boodd cells added into 2 000 μL was not statistically significant. Choosing 5 minutes is the appropriate time of centrifugation. Conclusion There are much influencing factor in the centrifugal specimen in examining HbA1c% ,including the specimen centrifuge,using Pipette and draw specimen links and so on . The volume of specimen and time of eentrifugation should be strictly controled.
出处
《检验医学与临床》
CAS
2013年第A02期35-36,共2页
Laboratory Medicine and Clinic
关键词
糖化血红蛋白
相关性
影响因素
Glyeated hemoglobin
relative
Influencing factors
