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塞来昔布减轻糖尿病大鼠心肌细胞纤维化的实验研究 被引量：1

Experimental study of celecoxib alleviating diabetic myocardial fibrosis in rats
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摘要 目的探讨塞来昔布对糖尿病心肌病大鼠心肌细胞纤维化的影响。方法将Wistar大鼠30只随机分为对照组(A组)、糖尿病心肌病组(B组)和糖尿病心肌病塞来昔布治疗组(C组)各10只。取大鼠心肌组织,经常规石蜡切片、染色后测定胶原纤维面的密度值;采用RT-PCR法测定TGF-β1mRNA表达情况。结果与A组相比,B组大鼠心肌组织的胶原纤维密度值、TGF-β1mRNA表达明显升高(P<0.05)。C组大鼠的胶原纤维、TGF-β1mRNA表达明显低于A组和B组(均P<0.05)。结论塞来昔布可能通过抑制TGF-β1减轻糖尿病大鼠心肌纤维化。 Objective To investigate the effect of celecoxib on myocardial fibrosis in diabetic rats. Methods Wistar rats were randomly divided into normal control group （A group, n= 10）, diabetic myocardial group （B group, n= 10） and diabetic cardiomyopathy celecoxib treatment group （C group, n= 10）. The rats were sacrificed and myocardial tissues underwent paraffin and staining tissue sections, the surface density of collagen fibers level was detected. The expression of transforming growth factor-βl （TGF-β1） mRNA was detected by RT-PCR. Results Compared with the normal control group, the collagen fibers of myocardial tissue in diabetic rats, TGF-β1 mRNA expression was significantly increased （P〈0. 05）. The collagen fibers in celecoxib treated rats, TGF-β1 mRNA expression was significantly lower than the diabetic myocardial group and normal control group （P〈0. 05）. Conclusion Celecoxib can alleviate development of myocardial fibrosis in diabetic rats.
作者 李若男 温俊平 林桂芳 吕琦 杨小玲 陈宁
机构地区 福建医科大学教学医院福建省老年医院内分泌科
出处 《福建医药杂志》 CAS 2013年第6期54-56,共3页 Fujian Medical Journal
基金 福建省卫生厅青年科研基金资助项目(2009-2-56)
关键词 塞来昔布 糖尿病心肌病 胶原纤维 转化生长因子-Β1 celecoxib diabetic cardiomyopathy collagen fiber TGF-β1
分类号 R587.2 [医药卫生—内分泌]
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参考文献9

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共引文献33

同被引文献30

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福建医药杂志
2013年 第6期

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