摘要
背景:富血小板血浆内含有的生长因子能够促进骨-腱愈合,从而减少了前交叉韧带重建后骨隧道扩大的发生;不同浓度的富血小板血浆对骨隧道的扩大有一定的影响,但是目前尚不清楚何种浓度更佳。目的:观察富血小板血浆对前交叉韧带重建后隧道扩大的影响。方法:将20只新西兰大白兔,参照Martinek等的方法进行兔的双侧前交叉韧带重建。在一侧肢体的肌腱移植物给予10 mg富血小板血浆凝胶预处理作为观察组,对侧肢体移植物给予5 mg富血小板血浆凝胶预处理为对照组。处理后12周进行影像学观察以及测量骨隧道的宽度,计算隧道扩大率。结果与结论:在前交叉韧带重建后的8周、12周的时间点上,10 mg富血小板血浆组隧道扩大的宽度小于5 mg富血小板血浆组(P<0.05);10 mg富血小板血浆组的腱-骨结合部位的骨密度值大于5 mg富血小板血浆组(P<0.05)。提示富血小板血浆能够促进兔前交叉韧带重建后腱-骨界面骨密度的提高,有效预防骨隧道的扩大,且以富血小板血浆浓度10 mg为佳。
BACKGROUND： Platelet-rich plasma contains growth factors that can promote tendon-bone healing, thereby reducing occurrence of bone tunnel enlargement after anterior cruciate ligament reconstruction; different concentrations of platelet-rich plasma have certain influence on bone tunnel enlargement, but it is not clear which concentration is optimal. OBJECTIVE： To study the effect of platelet-rich plasma on tunnel enlargement after anterior cruciate ligament reconstruction. METHODS： Bilateral anterior cruciate ligament reconstruction was conducted in 20 New Zealand white rabbits using Martinek et al＇s method. Tendon graft was pretreated with 10 mg platelet-rich plasma gel on one side as an observation group. Graft on the contralateral limb was pretreated with 5 mg platelet-rich plasma gel as control group. At 12 weeks after surgery, radiological observation and measure of the width of bone tunnel were conducted. Tunnel enlargement rate was calculated. RESULTS AND CONCLUSION： At 8 and 12 weeks after anterior cruciate ligament reconstruction, the width of tunnel enlargement was less in the 10 mg-platelet-rich plasma group than that in the 5 mg-platelet-rich plasma group （P 〈 0.05）. Bone density of tendon-bone interface was larger in the 10 mg-platelet-rich plasma group than that in the 5 mg-platelet-rich plasma group （P 〈 0.05）. These results indicated that platelet-rich plasma promoted in bone density of tendon-bone interface after rabbit anterior cruciate ligament reconstruction, effectively prevented enlargement of bone tunnel. Moreover, 10 mg platelet-rich plasma is perfect.
出处
《中国组织工程研究》
CAS
CSCD
2013年第50期8666-8671,共6页
Chinese Journal of Tissue Engineering Research
基金
沈阳医学院课题(20102040)
沈阳市卫生局课题(2011年青年基金)~~
