摘要
目的观察血府逐瘀汤对动脉血栓模型大鼠血浆中组织型纤溶酶原激活物(t-PA)、纤溶酶原激活物抑制剂-1(PAI-1)、纤溶酶原(PLG)含量和t-PA/PAI-1比值的影响。方法 SD大鼠60只,雌雄各半,随机分为空白组、模型组、阳性对照组、血府逐淤汤高、中、低剂量组,连续灌胃给药14 d,空白组和模型组予以生理盐水,用三氯化铁外敷颈总动脉复制血栓模型,观察血栓质量和测定血浆中t-PA、PAI-1、PLG含量。结果模型组颈总动脉有明显的血栓形成,说明造模成功;与模型组比较,药物组血栓质量均显著降低,其中血府逐瘀汤中剂量组血栓质量最轻,差异有统计学意义(P<0.01);与模型组比较,血府逐瘀汤能明显升高大鼠血浆中t-PA含量和t-PA/PAI-1比值、显著降低PAI-1、PLG含量,差异有统计学意义(P<0.01)。结论血府逐瘀汤通过升高t-PA含量、降低PAI-1、PLG含量,调节t-PA/PAI-1比值,达到纠正t-PA、PAI-1之间的平衡失调的作用。
Objective To observe the effects of Xuefu Zhuyu Decoction on the content of t- PA,PAI-1 ,PLG and the ratio of t-PA/PAI-1 in plasma of arterial thrombosis model rats. Methods 60 SD rats, half male and half female, were randomly divided into blank group, model group, posi- tive control group, XueFu ZhuYu Decoction high, medium and low dose group. The arterial throm- bosis model was replicated by external application with FeC13 at the carotid artery. Blank group and model group were irrigated with physiological saline, other rats were respectively fed up with drugs for 14 d. Thrombosis weight in each group was weighed. The content of PA, PAl -1 and PLG in plasma were measured by enzyme linked immunosorhent assay. Results Thrombosis oc- curred in carotid artery in model group. Compared with the model group, thrombus weight in drug groups were reduced markedly, and the thrombus weight est in all groups （P〈0.01）. Compared with the model group, t-PA plasma were significantly increased while the contents of were decreased in Xuefu Zhuyu clusion Xuefu Zhuyu Decoction Decoction groups, there were can adjust imbalance between of medium dose group was light- content and t-PA/PAI-1 ratio in PAI -1 and PLG in plasma significant differences PA content and reducing PAI-1 and PLG content and then leading PAI-1 ratio. t-PA and PAI-1 to the regulation （P〈0.01）. Con- by raising t- of the t-PA /
出处
《湖南中医药大学学报》
CAS
2013年第11期28-31,共4页
Journal of Hunan University of Chinese Medicine
基金
湖南省自然科学基金资助(2008031)
关键词
动脉血栓
纤溶活性
大鼠
血府逐瘀汤
Arterial thrombosis
fibrinolytic activity
rat
Xuefu Zhuyu Decoction
