摘要
目的测定马兰中多糖含量,为马兰的应用和质量评价提供科学依据。方法以葡萄糖为对照品,采用苯酚-硫酸比色法,在490nm波长下测定多糖含量。结果不同产地、采收时间的马兰多糖含量差异显著。结论苯酚-硫酸比色法操作简单,重复性好,结果准确可靠,可用于马兰中多糖的含量测定。
Objective To determine the content of polysaccharides in Kalimeris indica （Linn.） Sch. from different areas and to provide a scientific basis for the application and quality evaluation of Kalimeris indi- ca （Linn.） Sch. Methods With glucose as a control, the content of polysaccharides in Kalimeris indica （Linn.） Sch. from different areas was determined by phenol-sulfuric acid colorimetry at a wavelength of 490 nm. Results The content of polysaccharides varied significantly in Kalimeris indica （Linn.） Sch. pro- duced in different areas and harvested at different times. Conclusion This determination method has the advantages of easy operation, good repeatability, and accurate results and is suitable for content determi- nation of polysaccharides in Kalimeris indica （Linn.） Sch. from different areas.
出处
《安徽中医学院学报》
CAS
2013年第6期84-85,共2页
Journal of Anhui Traditional Chinese Medical College
基金
国家自然科学基金项目(81303220)
安徽省自然科学基金项目(11040606W220)
康缘中医药科技创新基金项目(KYCX 201003)
关键词
马兰
多糖
苯酚-硫酸比色法
Kalimeris indica （Linn.） Sch. ~ Polysaccharides~ Phenol-sulfuric acid colorimetry
