摘要
目的探讨胶质源性神经营养因子（glial cell line-derived neural factor,GDNF）基因修饰的神经干细胞（neural stem cells,NSCs）移植对大鼠脑缺血后（glial fibrillary acidic protein,GFAP）的表达的影响。方法建立大鼠暂时性大脑中动脉梗塞模型（middle cerebral artery occlusion,MCAO）,再灌注3d,并将其随机分为：生理盐水移植组（NS）、神经干细胞移植组（NSCs）和GDNF基因修饰的神经干细胞移植组（GDNF/NSCs）。采用脑立体定位仪将移植入大鼠的右侧脑室。再灌注1、2、3、5、7周的各时间点,处死大鼠,取同侧大脑半球,用Western blotting观察GFAP的表达。结果 GDNF/NSCs组、NSCs组和NS组GFAP蛋白均在2周达到高峰,后逐渐降低。而再灌注1~7周,GDNF/NSCs组的GFAP蛋白显著高于NS组（P〈0.001）;GDNF/NSCs组GFAP蛋白显著高于NSCs组（P〈0.01）。再灌注1~5周,NSCs组GFAP蛋白显著高于NS组（P〈0.001）。结论 GDNF/NSCs移植治疗大鼠缺血再灌注的神经保护作用,其机制可能与促进GFAP的表达有关。
Objective To investigate the effect of transplantating neural stem cells（neural stem cells,NSCs） modified by glial cell line-derived neural factor（GDNF） gene on expression of glial fibrillary acidic protein（GFAP） in rats with cerebral ischemia.Methods The cerebral ischemia model was performed by occluding middle cerebral artery for 2 hour and reperfusiong.At 3 days of reperfusion,saline,NSCs and GDNF / NSCs were administratied by a stereotaxic frame into right lateral ventricle.Then the rats were survived for 1,2,3,5 and 7W,respectively and followed to be sacrificed.Western blot was used to detect GFAP expression.Results GFAP expressions of GDNF / NSCs,NSCs and NS groups peaked at 2 weeks,then gradually decreased.At reperfusion 1 to 7 week,GFAP expressions of the GDNF / NSCs group were significantly higher than the NS group（P〈0.001）.At reperfusion 1 to 5 week,NSCs group GFAP expression was significantly higher than NS group（P〈0.001）.Moreover,GFAP expressions of GDNF / NSCs group was significantly higher than NSCs group（P〈0.01） at given time.Conclusion Transplantating GDNF / NSCs may increase GFAP expression in rats following ischemia,which may be one of the mechanisms that GDNF / NSCs tansplantaion provides the neruoprotection.
出处
《四川医学》
CAS
2013年第8期1099-1101,共3页
Sichuan Medical Journal
