摘要
从广西32家养殖场分别按100头以上母猪和100头以下母猪的不同规模猪场采集经过猪瘟疫苗一免、二免和三免后1个月的血清1083份,使用阻断ELISA方法对样品的猪瘟抗体水平进行检测。结果表明,一免和二免抗体合格率相比差异不显著,但和三免相比差异显著。不同免疫次数的抗体离散度差异不显著,100头母猪以上的猪场抗体离散度低于100头母猪以下的猪场。结果显示猪瘟抗体三免后效果最好,100头以下的猪场应加强对猪群猪瘟抗体的监测,根据个体的抗体消长规律进行有针对性的免疫,以提高群体抗体均匀度。
1083 pig serum samples were collected from 32 farms,16 of these farms had more than 100s ows and the other 16 had less than 100 sows.These serum samples were collected on 1 month after prime,first boost and second boost vaccinations with CSF vaccine,respectively.The antibody levels of these serum samples were measured by blocking-ELISA technique.The result showed that there is no significant difference in CSFV antibodies between the serums from the prime immunization and the first boost immunization,but the difference is significant compared with the second boost immunization.The antibody dispersion is obviously high in pig sera from farms less than 100 sows,while there is no significant difference in antibody dispersion after different immunizations.The result indicated that those pigs received the third immunization had optimal antibody levels.To reduce the antibody dispersion,those farms less than 100 sows should establish and optimize the vaccination procedure basing on the results of antibody surveillance in different herds of pigs.
出处
《动物医学进展》
CSCD
北大核心
2013年第12期244-246,共3页
Progress In Veterinary Medicine
关键词
猪瘟
免疫次数
抗体监测
离散度
CSFV
immunization times
antibody surveillance
dispersion
