摘要
在碱性介质中,发现血红蛋白对luminol-hydrazine弱化学发光体系有显著的增强作用。结合流动注射法提出了luminol-hydrazine化学发光(FI-CL)测定血红蛋白的新方法,优化了化学发光检测条件和FI-CL系统的运行参数,考察了实际样品中可能含有的共存物质对血红蛋白测定的影响。结果表明,血红蛋白的线性范围为5.0×10-9～6.0×10-5 g·mL-1,检出限是5.8×10-10 g·mL-1(9.0×10-12 mol·L-1),分别对5.0×10-7和3.0×10-6 g·mL-1的血红蛋白标准溶液平行八次测定所得RSDs分别为1.6%和1.5%。将提出的方法成功用于人血液和血清中血红蛋白含量的测定,加标回收率在83.0%～101.0%范围。结合化学发光光谱和紫外可见吸收光谱对血红蛋白催化luminol-hydrazine化学发光反应的机理进行了探讨。提出的方法具有灵敏度高、无需标记、操作简便、快速、易实现自动化操作等优点。
A sensitive method to detect hemoglobin （Hb） by flow injection （FI） coupled with chemilumines- cence （CL） detection was described, which based on Hb strong enhancing effect on weak luminol-hydrazine CL system in alkaline medium. Parameters affecting the CL detection conditions and FI-CL system were opti- mized. The effects of possible coexisting substances in human blood and serum of detection Hb were evalua- ted. Under the optimum conditions, the net CL intensity versus Hb concentration was linear in the range of 5.0 × 10^-9 6.0 × 10^-5 g. mL-1 with the detection limit of 5.8× 10^-10 g . mL-1 （9.0 × 10^-12mol . L-1 ）. The relative standard deviations （RSDs） for 8 replicate determinations of 5.0 ×10^-7 and 3.0 × 10^-6 g . mL-1 Hb were 1.6% and 1.5%, respectively. In addition, the recoveries of Hb in human blood and serum were car- ried out and varied from 83.0% to 101.0%. The proposed method has been successfully applied for the deter- mination of Hb in healthy human blood and serum. The possible mechanism of Hb enhancing the weak CL emission of luminol-hydrazine system in NaOH solution was discussed by fluorescence spectrophotometer and UV-Vis spectrophotometer.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2014年第1期241-245,共5页
Spectroscopy and Spectral Analysis
基金
The National Natural Science Foundation of China(21075106,21375114)
关键词
流动注射
化学发光
血红蛋白
联氨
Chemiluminescence
Flow-injection
Hemoglobin
Hydrazine
