摘要
通过PCR扩增126只布鲁氏菌阴性和67只布鲁氏菌阳性中国美利奴羊MHC-DRB1基因exon2,产物经SSCP电泳检测单核苷酸多态性(SNPs),而后对不同等位基因进行克隆测序,旨在确定该基因exon2多态位点,并对每个SNP位点的等位基因频率、基因型频率进行差异统计分析,从而分析其与布鲁氏菌病易感性的相关性。结果表明:在270 bp的序列内共检测到41个SNPs,其中109 C>T位点的等位基因在病例-对照样本中的分布存在显著差异(P<0.05)。由此表明,MHC-DRB1 exon2多态性与绵羊种布鲁氏菌病易感性显著相关。
The ovine major histocompatibility exon2 complex class H DRB1 exon2 was amplified by polymerase chain reaction （PCR）from 126 healthy and 67 infection with Brucellosis in Chinese Merino Sheep. PCR products were characterized by the SSCP technique, and then different alleles were cloned and sequenced to determine the gene polymorphism loci. Each SNP loci and differences in allele frequency and genotype frequency were analyzed statistlly, to analyze its correlation with brucellosis susceptibility. There were 41 single nucleotide polymorphisms （SNPs） in 270 bp DNA sequence. The distribution of 109 C〉T alleles had a significant difference in case-control samples. The results showed that the polymorphism of MHC-DRB1 gene exon2 was significant associated with Brucellosis susceptibility.
出处
《中国畜牧杂志》
CAS
北大核心
2014年第1期15-20,共6页
Chinese Journal of Animal Science
基金
国家重点基础研究发展计划(973计划)(2010CB530200)
