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基质辅助激光解吸／电离飞行时间质谱仪在醋酸钙鲍曼不动杆菌复合群鉴定中的应用研究 被引量：7

Species identification of Acinetobacter calcoaceticus-Acinetobacter baumannii complex by matrix- assisted laser desorption/ionization time-of-flight mass spectrometry
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摘要 目的评价基质辅助激光解吸／电离飞行时间质谱（MALDI—TOFMS）对醋酸钙鲍曼不动杆菌复合群的鉴定价值，了解浙江大学医学院附属第二医院醋酸钙鲍曼不动杆菌复合群的菌种分布情况。方法回顾性选取2012年1至7月我院临床分离的502株醋酸钙鲍曼不动杆菌复合群非重复菌株，用MALDI—TOFMS进行再鉴定，同时用16S-23SrRNA基因间隔区序列分析来确定所有菌株的菌种，比较二者鉴定结果的差异。结果502株醋酸钙鲍曼不动杆菌复合群用MALDI—TOFMS再鉴定，包括鲍曼不动杆菌431株（85．9％），皮特不动杆菌68株（13．5％），醋酸钙不动杆菌3株（0．6％）；对502株醋酸钙鲍曼不动杆菌复合群16S-23SrRNA基因间隔区序列分析结果显示：403株为鲍曼不动杆菌（80．3％），68株为皮特不动杆菌（13．5％），28株为医院不动杆菌（5．6％），3株为醋酸钙不动杆菌（0．6％）。与16S-23SrRNA基因间隔区序列分析相比较，MALDI—TOFMS错误地将所有28株医院不动杆菌鉴定为鲍曼不动杆菌，其余菌株的鉴定结果完全一致。结论MALDI—TOFMS可将醋酸钙鲍曼不动杆菌复合群菌株准确鉴定到种，具有操作简便、快速、成本低，重复性好的优点。MALDI—TOFMS有望在未来成为日常临床微生物鉴定的理想工具。 Objective To evaluate the ability of matrix-assisted laser desorption/ionization time-of- flight mass spectrometry （MALDI-TOF MS） in identifying species of Acinetobacter calcoaceticus-Acinetobacter baumannii complex, and investigate the species distribution of Acinetobacter calcoaceticus-Acinetobacter baurnannii complex isolated in our hospital. Methods A total of 502 nonduplicate clinical isolates of Acinetobacter calcoaceticus-Acinetobacter baumannii complex were retrospectively collected from the second affiliated hospital of Zhejiang University between January 2012 and July 2012. All strains were re-identified by MALDI-TOF MS and were also verified by sequence analysis of 16S-23S rRNA gene spacer region. Results Among all the 502 strains of Acinetobacter calcoaceticus-Acinetobacter baumannii complex, identificaion results provided by MALDI-TOF MS were A. baumannii （431, 85.9%）, A. pittii （68, 13.5% ） , A. calcoaceticus （3, 0. 6% ）. Sequence analysis of 16S-23S rRNA gene spacer region was used to identify all the 502 strains： 403 （80. 3% ） were identified as A. baumannii, 68 （13.5%） as A. pittii, 28 （5.6%） as A. nosocomialis and 3 （0.6%） as A. calcoaceticus. MALDI-TOF MS correctly identified all the strains but erroneously identified all 28 strains of A. nosocomialis as A. baumannii, compared with sequence analysis of 16S-23S rRNA gene spacer region. Conclusions MALDI-TOF MS can be used as a fast, simple, reliable and excellently reproducible method to identify members of Acinetobacter calcoaceticus- Acinetobacter baumannii complex at low costs. MALDI-TOF MS is expected to be an ideal technique for routine clinical microbiology testing in the future.
作者 吴伟根 黄永禄 杨旭峰 周宏伟 张嵘
机构地区 浙江大学医学院附属义乌医院检验科 浙江大学医学院附属第二医院检验科
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2013年第12期1115-1119,共5页 Chinese Journal of Laboratory Medicine
关键词 不动杆菌 乙酸钙 鲍氏不动杆菌 光谱法 质量 基质辅助激光解吸电离 Acinetobacter calcoaceticus Acinetobacter baumannii Spectrometry, mass, matrix-assisted laser desorption-ionization
分类号 R446.5 [医药卫生—诊断学]
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参考文献18

  • 1Munoz-Price LS,Weinstein RA. Acinetobacter infection[J].{H}New England Journal of Medicine,2008.1271-1281.
  • 2Turton JF,Kaufmann ME,Warner M. A prevalent,multiresistant clone of Acinetobacter baumannii in Southeast England[J].{H}Journal of Hospital Infection,2004.170-179.
  • 3王辉,郭萍,孙宏莉,杨启文,陈民钧,朱元珏,徐英春,谢秀丽.碳青霉烯类耐药的不动杆菌分子流行病学及其泛耐药的分子机制[J].中华检验医学杂志,2006,29(12):1066-1073. 被引量：237
  • 4Gerner-Smidt P,Tjernberg I,Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species[J].{H}Journal of Clinical Microbiology,1991.277-282.
  • 5Idzenga D,Schouten MA,van Zanten AR. Outbreak of Acinetobacter genomic species 3 in a Dutch intensive care unit[J].{H}Journal of Hospital Infection,2006.485-487.
  • 6Chuang YC,Sheng WH,Li SY. Influence of genospecies of Acinetobacter baumannii complex on clinical outcomes of patients with acinetobacter bacteremia[J].{H}Clinical Infectious Diseases,2011.352-360.
  • 7Lee NY,Chang TC,Wu C J. Clinical manifestations,antimicrobial therapy,and prognostic factors of monomicrobial Acinetobacter baumannii complex bacteremia[J].{H}Journal of Infection,2010.219-227.
  • 8van Veen SQ,Claas EC,Kuijper EJ. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories[J].{H}Journal of Clinical Microbiology,2010.900-907.
  • 9Bizzini A,Durussel C,Bille J. Performance of matrixassisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory[J].{H}Journal of Clinical Microbiology,2010.1549-1554.
  • 10Cai JC,Hu YY,Zhang R. Detection of OmpK36 porin loss in Klebsiella spp.by matrix-assisted laser desorption ionizationtime of flight mass spectrometry[J].{H}Journal of Clinical Microbiology,2012.2179-2182.

二级参考文献18

  • 1王辉,孙宏莉,廖康,陈冬梅,王清涛,王飞燕,陈民钧,朱元珏.北京和广州地区四家医院不动杆菌碳青霉烯酶基因型研究[J].中华检验医学杂志,2005,28(6):636-641. 被引量：121
  • 2Manikal VM,Landman D,Saurina G,et al.Endemic carbapenemresistant Acinetobacter species in Brooklyn,New York:citywide prevalence,interinstitutional spread,and relation to antibiotic usage.Clin Infect Dis,2000,31:101-106.
  • 3Kuo L,Teng L,Yu C,et al.Dissemination of a clone of unusual phenotype of pandrug-resistant Acinetobacter baumannii at a university hospital in Taiwan.J Clin Microbiol,2004,42:1759-1763.
  • 4Clinical and Laboratory Standards Institute.2005.Performance standards for antimicrobial susceptibility testing; fifteenth informational supplement.Clinical and Laboratory Standards Institute,Wayne,Pa.
  • 5Tenover FC,Arbeit RD,Goering RV,et al.Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis:criteria for bacterial strain typing.J Clin Microbiol,1995,33:2233-2239.
  • 6Lévesque C,Piché L,Larose C,et al.PCR mapping of integrons reveals several novel combination of resistance genes.Antimicrob Agents Chemother,1995,39:185-191.
  • 7Garner JS,Jarvis WR,Emori TG,et al.CDC definitions for nosocomial infections.Am J Infect Control,1988,16:128-140.
  • 8Rello J.Acinetobacter baumannii infections in the ICU:customization is the key.Chest,1999,115:1226-1229.
  • 9Rodriguez-Bano J,Cisneros JM,Fernandez-Cuenca F,et al.Clinical features and epidemiology of Acinetobacter baumannii colonization and infection in Spanish hospitals.Infect Control Hosp Epidemiol,2004,25:819-824.
  • 10Dalla-Costa LM,Coelho JM,Souza HAPHM,et al.Outbreak of carbapenem-resistant Acinetobacter baumannii producing the OXA-23enzyme in Curitiba,Brazil.J Clin Microb,2003,41:3403-3406.

共引文献236

同被引文献35

  • 1Seng P, Drancourt M, Gouriet F bacteriology : routine identification et al. Ongoing revolution in of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry [ J ], Clin Infect Dis, 2009, 49(4) : 543-551.
  • 2Fenselau C, Demirev PA. Characterization of intact microorganisms by MALDI mass spectrometry[ J]. Mass Spectrom Rev, 2001, 20(4) : 157-171.
  • 3Kumar MP, Vairamani M, Raju NP, et al. Rapid discrimination between strains of beta haemolytic streptococci by intact cell mass spectrometry[J]. Indian J Med Res, 2004,119(6) : 283-288.
  • 4Lartigue MF, Hrry-Arnand G, Haguenoer E, et al. Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization time-of-flight mass spectrometry[ J]. J Clin Microbiol,2009, 47 (7) : 2284- 2287.
  • 5Williamson Y, Moura H, Woolfitt A, et al. Differentiation of Streptococcus pneumoniae conjunctivitis outbreak isolates by matrix-assisted laser desorption ionization time of flight mass spectrometry[J]. J Clin Microbiol,2008, 74(19) : 5891-5897. Illina E, Borovskaya A, Malakhova M, et al. Direct bacterial profiling by matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria[J]. J Mol Diagn ,2009,11 ( 1 ) : 75-86. Chao QT,Lee TF, Teng SH,et al. Comparison of the accuracy of two conventional phenotypic methods and two MALDI-TOF MS systems with that of DNA sequencing analysis for correctly identifying clinically encountered yeasts [ J ] ['los One, 2014,9 (10) :e109376. Majchrzykiewicz-Koehorst JA, Heikens E, Trip H, et al. Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry[ J ]. J Virol Methods, 2015,213 : 75 -83. Rettinger A, Krupka I, Grtinwald K, et al. Leptospira spp. strain identification by MALDI TOF MS is an equivalent tool to 16S rRNA gene sequencing and multi locus sequence typing (MLST) [Jl. BMC Mierobiol, 2012,12:185. Fujinami Y, Kikkawa H, Kurosaki Y,et al. Rapid discrimination of Legionella by matrix-assisted laser desorption ionization time-of- flight mass spectrometry [ J ]. Microbiol Res, 2011,166 (2) : 77- 86. Bittar F, Ouchenane Z, Smati F, et al. MALDI - TOF - MS for rapid detection of staphylococcal Panton - Valentine leukocidin . Int Antimicrob Agents, 2009,34(5) :467-470. Lu JJ, Tsai FJ, Ho CM, et al. Peptide biomarker discovery for identification of methicillin-resistant and vancomycin-intermediate Staphylococcus aureus strains by MALDI-TOF [ J ]. Anal Chem, 2012, 84(13) : 5685-5692. Schuhhess B, Brodner K, Bloemberg GV, et al. Identification of Gram-positivecocci using MALDI-TOF MS : comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics [ J]. J Clin Microbiol, 2013, 51 (6) : 1834-1840.
  • 6Williamson Y, Moura H, Woolfitt A, et al. Differentiation of Streptococcus pneumoniae conjunctivitis outbreak isolates by matrix-assisted laser desorption ionization time of flight mass spectrometry[J]. J Clin Microbiol,2008, 74(19) : 5891-5897.
  • 7Illina E, Borovskaya A, Malakhova M, et al. Direct bacterial profiling by matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria[J]. J Mol Diagn ,2009,11 ( 1 ) : 75-86.
  • 8Chao QT,Lee TF, Teng SH,et al. Comparison of the accuracy of two conventional phenotypic methods and two MALDI-TOF MS systems with that of DNA sequencing analysis for correctly identifying clinically encountered yeasts [ J ] ['los One, 2014,9 (10) :e109376.
  • 9Rettinger A, Krupka I, Grtinwald K, et al. Leptospira spp. strain identification by MALDI TOF MS is an equivalent tool to 16S rRNA gene sequencing and multi locus sequence typing (MLST) [J]. BMC Microbiol, 2012,12:185.
  • 10Fujinami Y, Kikkawa H, Kurosaki Y,et al. Rapid discrimination of Legionella by matrix-assisted laser desorption ionization time-of- flight mass spectrometry [ J ]. Microbiol Res, 2011,166 (2) : 77- 86.

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中华检验医学杂志
2013年 第12期

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