摘要
目的:研究原发性干燥综合征患者唇腺中细胞色素C表达水平的改变。方法:选择原发性干燥综合征患者35例和健康对照15例,采用免疫组化法和反转录-聚合酶链式反应(RT-PCR)检测2组唇腺组织中CytC蛋白和mRNA的表达,测量吸光度值,采用SPSS14.0软件包对结果进行Mann-Whitney检验,并与临床检验结果进行Spearman相关分析。结果:原发性干燥综合征患者唇腺组织中CytC在蛋白水平和mRNA水平均高表达,与健康对照组相比显著升高(P<0.05),并与疾病的病程呈正相关。随着唇腺淋巴细胞浸润灶等级的增加,CytC的表达也随之升高(P<0.05),但与其他临床特征之间不存在明显相关性。结论:CytC在原发性干燥综合征患者唇腺组织中表达增强,且与病理分级和疾病的病程相关。
PURPOSE： To explore the expression of CytC in the labial glands of patients with primary Sjogren＇s syndrome （pSS）. METHODS： Thirty-five cases with pSS and 15 controls were collected. Immunohistochemistry and RT- PCR were used to examine CytC protein and mRNA in labial glands of 2 groups. The integral optical density were measured and the data was analyzed by Mann-Whitney test with SPSS14.0 software package. The correlation between the clinical manifestations and the expression of CytC were calculated by Spearman＇s analysis. RESULTS： The expression of CytC protein and mRNA in pSS were positively correlated with the disease course and significantly higher than those in control group （P〈0.05）. There was a positive correlation between the lymphocyte focus score and the expression of CytC, but no correlation with the other clinical parameters. CONCLUSIONS： The expression of CytC in pSS is increased,which is related to the disease course and the lymphocyte focus score. Supported by Science and Technology Research Project of Liaoning Province （2011225020） and College Scientific Research Foundation for Youth in 2013 （K101593-13-39）.
出处
《上海口腔医学》
CAS
CSCD
北大核心
2013年第6期680-683,共4页
Shanghai Journal of Stomatology
基金
辽宁省科学技术计划项目(2011225020)
2013年院级青年科研启动基金(K101593-13-39)~~
关键词
干燥综合征
唇腺
凋亡
细胞色素C
SjOgren ＇ s syndrome
Labial gland
Apoptosis
CytC
