摘要
利用蚕豆病毒属的兼并引物对23个加工番茄病株进行RT-PCR检测,其中19株检测出被该属病毒侵染。从中选取4个病株的RT-PCR产物进行克隆和测序,将所得序列经过基因库检索发现,4个序列均与蚕豆萎蔫病毒2(BBWV2)基因组RNA1的序列同源性最高。然后对病毒分离物XJ14-1基因组进行进一步的克隆和测序,获得RNA1组分3659个核苷酸,RNA2组分1300个核苷酸,序列比对结果显示,它们与BBWV2的RNA1和RNA2具有高的序列同源性,分别为76.6%~94%和76.9%~94.1%,而与BBWV1的RNA1和RNA2的序列相似性都很低。因此分子鉴定结果表明,新疆加工番茄受到BBWV2的侵染。利用BBWV2的单克隆抗体对田间不同品种(品系)加工番茄病株进行ELISA检测,结果显示田间病株带毒率达到53.3%,并且供试20品种(品系)均可被BBWV2侵染,该数据表明,BBWV2在田间发生普遍。
Processing tomato plants showing viral symptom were detected by RT-PCR using universal primers of Fabavirus to determine virus species. Fragments of about 400 bp were amplified from 19 out of 20 samples,and RT-PCR products of 4 sampies were chosen to clone and sequence. 4 sequences shared high nucleotide sequence identities with RNA1 of BBWV2. Isolate XJ14-1 was chosen to determine genomic RNA1 and RNA2. Partial RNA1 of 3659 nucieotides and partial RNA2 of 1300 nuc[e otide were determined. Sequence comparison showed that RNA1 and RNA2 of XJ14-1 had 76.6G^94~ and 76.9%~94.1G sequence identities with those of BBWV2 isolates, respectively, while they shared low identities with Broad bean wilt virus 1 （BBWV1）. It was concluded that processing tomato plants showing viral symptom were infected by BBWV2 in Xinjiang. Field samples of processing tomato showing viral symptom were detected by ELISA using monoclonal antibodies （MAbs） against BB- WV2. The results showed that incidence rate of plants infected by BBWV2 were 53.3%,and 20 tested cuhivars could be infec- ted by BBWV2. It indicates that BBWV2 occurs widely in the field.
出处
《石河子大学学报（自然科学版）》
CAS
2013年第6期675-679,共5页
Journal of Shihezi University(Natural Science)
基金
石河子大学自然科学创新团队项目(2011ZRKXTD-02)
