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基质辅助激光解吸电离飞行时间质谱技术在临床微生物鉴定中的应用及价值 被引量：12

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摘要 20世纪90年代末,基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)技术开始用于病原菌鉴定,目前已逐步用于临床微生物检验工作,对临床感染性疾病的快速诊断意义重大。本文阐述MALDI-TOF MS技术鉴定微生物的原理和流程、在临床微生物鉴定中的应用和对微生物检验结果回报时间(TAT)改进的作用。
作者 顾兵 李永军
机构地区 南京医科大学第一附属医院检验学部 梅里埃诊断产品上海有限公司
出处 《临床检验杂志》 CAS CSCD 北大核心 2013年第11期814-817,共4页 Chinese Journal of Clinical Laboratory Science
关键词 基质辅助激光解吸电离飞行时间 质谱 回报时间 微生物 感染性疾病
分类号 R446.5 [医药卫生—诊断学]
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二级参考文献44

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共引文献14

同被引文献80

  • 1蒋娟娟,冯芳.基质辅助激光解吸离子化中的基质和基质添加剂[J].药学进展,2004,28(8):349-354. 被引量：8
  • 2张淑红,吴清平,张菊梅,郭伟鹏.显色培养基在几种食源性致病菌快速检测中的应用[J].微生物学通报,2006,33(6):108-111. 被引量：39
  • 3许永建,罗荣辉,郭茂田,吴俊富,李明玉.共聚焦显微拉曼光谱的应用和进展[J].激光杂志,2007,28(2):13-14. 被引量：31
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  • 9Nagy E,Maier T, Urban E,et al. Species identification of clinical i-solates of Bacteroides by matrix-assisted laserdesorption/ionizationtime-of-flight mass spectrometry [ J ]. Clin Microbiol Infect, 2009 ,15(8):796-802.
  • 10Gamer 0,Mochon A,Branda J, et al. Multi-centre evaluation ofmass spectrometric identification of anaerobic bacteria using theVITEK. MS system [ J ]. Clin Microbiol Infect, 2014,20(4):335-339.

引证文献12

二级引证文献70

临床检验杂志
2013年 第11期

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