摘要
目的检测大肠癌患者根治术中植入氟尿嘧啶植入剂后外周血survivin、caspase-3、CD44V6的变化,探讨其对肿瘤微转移及预后的影响。方法 64例大肠癌患者(Dukes B期、C期)随机分为治疗组和对照组,每组32例。2组均行标准根治术,治疗组术中植入氟尿嘧啶植入剂;对照组术中未应用此药。采用RT-PCR方法测定手术前后外周血中survivin、caspase-3表达水平,流式细胞术检测CD44V6含量。结果术前2组survivin、caspase-3、CD44V6的水平差异无统计学意义(P>0.05);术后14 d,治疗组survivin水平(0.362±0.183)低于对照组(0.585±0.207),caspase-3水平(2.001±0.146)高于对照组(1.654±0.111);术后CD44V6水平治疗组(1.857±0.535)和对照组(3.471±0.496)明显低于术前的(9.557±1.170)和(9.729±0.943),且治疗组CD44V6水平低于对照组(均P<0.05)。结论术中植入氟尿嘧啶植入剂,对大肠癌的微转移及预后有重要意义,有望提高大肠癌术后的远期疗效。
Objectives To observe the pre and post-operational changes of the expressions of survivin, caspase-3 and CD44V6 in patients with eoloreetal cancer after intra-abdominal implantation of sustained releasing fluorouraeil. Meth- ods Sixty-four patients with colorectal cancer （Dukes＇ stage of B and C） were divided into treatment group and control group, 32 patients in each group. The standard radical surgery was performed in two groups of patients. The fluorouracil im- plants were implanted intra-abdominally in treatment group. The peripheral l^lood levels of surviving and caspase-3 were de- tected by RT-PCR. The level of CD44V6 was detected by flow cytometry in two groups of patients. Results There were no significant differences in levels of survivin, easpase-3 and CD44V6 before surgery between two groups （P 〉 0.05）. The level of survivin （0.362＋0.183） was significantly lower at 14 days after operation in treatment group than that of control group （0.585-＋0.207）, but the level of caspase-3 （2.001＋0.146） was significantly higher than that of control group （1.654：t：0.111）. The levels of CD44V6 were significantly lower in treatment group （1.857＋0.535） and control group （3.471＋0.496） after opera- tion than those before operation （9.557＋ 1.170 and 9.729＋0.943, P 〈 0.05）, and the level of CD44V6 was significantly lower in treatment group than that of control group （P 〈 0.05）. Conclusion The implant for the sustained release of fluorouracil showed a positive impact on mierometastases and prognosis of colorectal cancer, while improved the long-term efficacy of postoperative colorectal cancer.
出处
《天津医药》
CAS
北大核心
2014年第1期7-9,I0002,共4页
Tianjin Medical Journal
基金
广西壮族自治区卫生厅计划项目(项目编号:22012370)
