摘要
采用PMP柱前衍生化HPLC对水解后银杏外种皮多糖中的单糖组分进行研究。采用Agilent HC-C18色谱柱(4.6 mm×250 mm,5μm);流动相0.1 mol·L-1磷酸盐缓冲液(pH 6.8)-乙腈(84∶16)等度洗脱;柱温40℃,流速1 mL·min-1,检测波长245 nm。从银杏外种皮多糖水解液中分析鉴定了6种单糖,分别为甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖,其摩尔比为0.032∶0.14∶0.296∶0.403∶0.106∶0.046。
A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone （PMP） has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-Cls column （4. 6 mmx 250 mm,5 p,m）, optimized for the separation of PMP derivatives, was used at ambient temperature of 40 ℃. The PMP deriva- tives elution was performed with a mixture of 0. 1 mol · L-1 phosphate buffer （pH 6. 8） and acetonitrile in a ratio of 84：16 at a flow rate of 1 mL · min^- 1, and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaeeharides mainly containing mannose, rhamnose, D-galacturonie acid, glucose, ga- lactose, arabinose, with the molar ratio of 0. 032： 0. 14： 0. 296： 0. 403： 0. 106： 0. 046.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第2期262-266,共5页
China Journal of Chinese Materia Medica
基金
江苏省科技发展计划医药高技术研究基金项目(BG2007609)
关键词
银杏
多糖
单糖
PMP
HPLC
Ginkgo biloba
polysaccharides
monosaccharide
PMP
HPLC
