摘要
从广东省某商品代肉鸡场12日龄生长迟缓的小鸡体内分离到1株鸡传染性贫血病毒（CAV），暂定名为GI）_1—12株。分离病毒用MDCC—MSB1细胞进行培养，通过肌肉注射方式感染1日龄SPF鸡，于感染后第7天、第14天、第21天分别检测CAV对SPF鸡体质量、胸腺、脾脏、肝脏、法氏囊及胸腺和外周血液中CD3＋T淋巴细胞指标的影响，同时观察鸡的临床症状。结果表明，GD-1—12分离株导致SPF鸡30％的死亡率。感染后第14天，胸腺极度萎缩，肝脏肿大，法氏囊萎缩，脾脏萎缩。感染后第7～14天之间，胸腺和外周血中CD3＋T淋巴细胞呈下降趋势。结果表明1日龄SPF雏鸡在人工感染CAV后第7～14天是损伤上述器官的关键时期。
A field strain GD-l-12 of chicken anemia virus （CAV） was isolated from a 12-day-old broiler flock with growth runting syndromes from Gongdong province. MDCC-MSB1 cells were used to proliferate the virus of GD-l-12 isolate. SPF chickens at 1 day were challenged with the virus by intramuscular injection. Ratios of thymuses, spleens, livers, the bursas to body weight, and the CD3＋ T cells in thymuses and pe- ripheral blood were analyzed after infection at 7, 14, 21-day. Observation of clinical symptoms at 7, 14, 21-day after infections with CAV. Results indicated that the GD-I-12 isolate caused high mortality about 30%, belonging to highly pathogenic. After infection at 14 day, resulting in severe thymic atrophy, hepa- tomegaly, bursa atrophy, spleen atrophy. After challenging at 7-14 day, the CD3＋ T cells in thymuses and peripheral blood were significantly decreased, it indicated that T lymphocytes were the target cells. The research further illustrated that after infection at 7-14 day was the critical period of the immune func- tion with the serious obstacle.
出处
《动物医学进展》
CSCD
北大核心
2014年第1期27-31,共5页
Progress In Veterinary Medicine
基金
广东省重大科技项目(2009B020201008)
