摘要
目的:观察辛伐他汀对2型糖尿病大鼠心肌Toll样受体4(TLR4)表达的影响。方法:40只雄性SD大鼠随机分为对照组(n=10)和实验组(n=30)。其中对照组给予普通饲料,实验组用高糖高脂饲料喂养。6周后测定空腹血糖>7.8 mmol/L且伴有胰岛素敏感指数降低的大鼠作为2型糖尿病大鼠模型,共26只,将其随机分为糖尿病组(n=13)和辛伐他汀组(n=13)。干预治疗8周。留取心脏组织做光镜观察,分别采用免疫组织化学染色和逆转录多聚酶链反应法(RT-PCR)检测各组大鼠心肌TLR4蛋白和信使核糖核酸(mRNA)的表达。结果:与对照组比较,光镜下可见糖尿病组心肌细胞明显水肿,并可见大量炎细胞浸润,辛伐他汀组心肌组织损伤较糖尿病组明显减轻。免疫组织化学染色和RT-PCR显示,糖尿病组大鼠TLR4蛋白和mRNA表达分别为0.6157±0.0233和1.43±0.16,明显高于对照组(0.3210±0.0151和0.42±0.06,P<0.05);辛伐他汀组大鼠TLR4蛋白和mRNA表达分别是0.4302±0.0224和0.73±0.13,低于糖尿病组(P<0.05),但仍高于对照组(P<0.05),差异均有统计学意义。结论 :辛伐他汀从蛋白和mRNA水平下调2型糖尿病大鼠心肌TLR4的表达、降低炎症反应,进而发挥对心脏的保护作用。
Objective： To observe the effect of simvastatin on reducing the expression of myocardial Toll like receptor 4 （TLR4） in type 2 diabetic rats. Methods： A total of 40 male SD rats were divided into 2 groups, Control group, n=10, me rats had normal feed and Experimental group, n=30, the rats received hyperglycemia and hypelipaemia feed for 6 weeks. There were 26 experimental rats with fasting blood glucose 〉7.8 mmol/L defined as diabetic rats and divided into 2 groups, Diabetic group, the rats received the normal saline, and Simvastatin group, the rats received simvastatin 20mg/（kg·d） for 8 weeks, n=13 in each group. The myocardial tissue was examined by optical microscopy, TLR4 protein and mRNA expressions were measured by immuno histochemistry and RT-PCR respectively in different groups. Results： Compared with Control group, the myocardial tissue in Diabetic group showed obvious edema and inflammatory cell infiltration, those damages were much less in Simvastatin group. The immuno-histochemistry and RT- PCR presented that TLR4 protein and mRNA expressions in Diabetic group （0.6157 ± 0.0233） and （1.43 ± 0.16） were significantly higher than those in Control group （0.3210 ± 0.0151） and （0.42 ± 0.06） all P〈0.05. The TLR4 protein and mRNA expressions in Simvastatin group （0.4302 ± 0.0224） and （0.73 ± 0.13） were lower than Diabetic group but higher than Control group, all P〈0.05. Conclusion： Simvastatin may reduce the expressions of TLR4 protein and mRNA and decrease the inflammatory reaction therefore protect the cardiac tissue in experimental rats.
出处
《中国循环杂志》
CSCD
北大核心
2014年第1期64-67,共4页
Chinese Circulation Journal
