摘要
从江西井冈山地区采集的土壤样品中分离获得76株放线菌,通过对峙培养法筛选出菌株S15对香蕉枯萎病菌Fusarium oxysporum、芦笋茎枯病菌Phomopsis asparagi有强抑制作用,该菌培养滤液对稻瘟病菌Magnaporthe oryzae、番茄灰霉病菌Botrytis cinerea、大豆炭疽病菌Colletotrichum truncatum、水稻纹枯病菌Rhizoctonia solani、柑橘炭疽病菌Colletotrichum gloeosporioides、香蕉黑星病菌Cladosporium cucumerinum、番茄早疫病菌Alternaria solani、黄瓜炭疽病菌Colletotrichum orbiculare、玉米大斑病菌Setosphaeria turcica等多种重要的植物病原真菌也均有较强的抑制作用,拮抗谱较宽。根据其形态特征、培养特性、生理生化特性和16S rDNA序列同源性分析,S15被鉴定为链霉菌属波卓链霉菌Streptomyces bottropensis。
Seventy-six strains of actinomycete were isolated from the soil in Jinggangshan area in Jiangxi province. S15 was found to be strongly antagonistic to Fusarium oxysporum and Phomopsis asparagi. The inhibition effect of S15 fermentation filtrate was significant against Magnaporthe oryzae,Botrytis cinerea,Colletotrichum truncatum,Rhizoctonia solani,Colletotrichum gloeosporioides,Cladosporium cucumerinum,Alternaria solani,Colletotrichum orbiculare and Setosphaeria turcica. According to its morphology, cultural characteristics, physiological and biochemical characteristics and 16S rDNA sequence, S15 were identified to be Streptomyces bottropensis.
出处
《福建农业学报》
CAS
2013年第10期1027-1031,共5页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划项目--省属公益类科研院所基本科研专项(2011R1026-8)
福建省农业科学院青年科技人才创新项目(2011QB-16)
公益性行业(农业)科研专项(201003074-4-1
201003074-6-1)
关键词
香蕉枯萎病菌
芦笋茎枯病菌
放线菌
拮抗
Fusarium oxysporum, Phomopsis asparagi, actinomycete, antagonism
