摘要
目的:研究富勒烯(C60)对RNA逆转录(Reverse Transcription,RT)反应的影响。方法:以斑马鱼鱼肝mRNA为模板,在M-MLV逆转录酶作用下合成cDNA,并在RT反应体系中添加不同浓度的C60,分别研究C60对M-MLV逆转录酶和RNA构象的影响。结果:100μg/mL C60可显著抑制RT反应,抑制效应与C60浓度相关,但增加M-MLV逆转录酶量可以有效逆转C60对RT的抑制作用。C60与RNA在37℃避光孵育12 h后,C60可特异降解28S rRNA,同样具有浓度依赖性。结论:C60不仅可显著抑制M-MLV逆转录酶活性,而且对RNA也具有一定的损伤作用,C60具有潜在的基因毒性。
Objective： To elucidate the effects of fullerene （C60） on reverse transcription （RT）. Methods： Template mRNA was extracted from the liver of zebrafish, synthesised cDNA with M-MLV reverse transcriptase. Different concentrations of C60 were added into the RT reaction solu- tion, to study the impact of C60 on M-MLV reverse transcriptase and RNA conformation respectively. Results： The results showed that 100 μg/mL of C6o could significantly inhibit the RT reaction, and this effect was related to the C60 concentration. The RT inhibition of C60 could be reversed by increas- ing the amount of M-MLV reverse transcriptase in the RT reaction solution. After its co-incubation with RNA in 37 ℃ for 12 h without light, C60 can specifically degraded 28S rRNA in the concentra- tion-dependent manner. Conclusion： These results demonstrate that C60 could not only inhibit the activity of M-MLV reverse transcriptase, it also has damaging effect on the RNA. C6o may have po- tential genetics toxicity.
出处
《江汉大学学报（自然科学版）》
2013年第6期66-71,共6页
Journal of Jianghan University：Natural Science Edition
基金
国家自然科学基金(20907017
20890112)
