摘要
酶修饰技术可以提高酶的稳定性、催化效率、活力和延长半衰期等性质,使其更好的应用于当今食品、医药等行业。本文重点综述了蛋白质交联修饰、小分子化学修饰、辅助因子修饰等七种分子化学修饰方法和分子定向化修饰、定点突变修饰等三种基因工程修饰方法以及它们在食品、医药和化工行业的应用,并对酶修饰技术的发展前景进行了展望。
Enzymatic modification could improve its stability,catalytic efficiency,biological activity,mission life, and etc.,which promotes its application in food and medicine industries. This review covered seven chemical modification methods such as protein cross-linking modification,small-molecules chemical modification,and cofactor modification,and three genetic engineering methods such as molecular orientation modification and site-directed mutagenesis modification,as well as their applications in the food,pharmaceutical and chemical industries. Additionally,the prospects of enzymatic modification development also be highlighted.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第2期350-353,共4页
Science and Technology of Food Industry
基金
国家自然科学基金项目(311641/C200101)
河南省科技计划项目(122102110037)
关键词
酶修饰
化学修饰
基因工程
应用
enzymatic modification
chemical modification
genetic engineering
application
