摘要
以吉粳88为材料,通过对不同培养基和激素的筛选,确定最佳的再生体系。结果表明:NMB培养基为吉粳88最适的愈伤组织诱导培养基。通过L9(33)正交实验研究最适激素浓度配比,筛选出吉粳88愈伤组织诱导的最佳激素配比为2.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.10 mg/L NAA。在添加2.0 mg/L 6-BA+0.5 mg/L NAA的NMB基本培养基条件下,吉粳88的分化率最高,成培苗率也最高。
Jijing 88 was used as the research material and different media and hormone combinations were selected,in order to obtain its optimal in-vitro regeneration system. The results showed that NMB medium was the most suitable medium for rice callus induction among the 5 medium. The most suitable plant hormone ratio was investigated by using orthogonal test L9（33）,and the induction rate was the highest when 2.0 mg/L 2,4-D,0.2 mg/L 6-BA,0.10mg /L NAA were added. The differentiation rate and regeneration rate were the highest when 2.0 mg /L6-BA,0.5mg /L NAA were added.
出处
《广西植物》
CAS
CSCD
北大核心
2013年第6期865-869,共5页
Guihaia
基金
长春市科技计划项目(11kz77)
吉林省自然科学基金(201215181)
关键词
吉粳88
培养基
诱导率
分化率
成苗率
Jijing 88
culture medium
induction rate
differentiation rate
regeneration rate
