摘要
目的:建立紫草闪式提取醇提物的HPLC指纹图谱,全面完整地反映其内在化学信息,为紫草醇提物的质量控制提供依据。方法:采用高效液相色谱法,色谱柱为Kromasil 100-5 C18(4.6 mm×250 mm,5μm),以水-甲酸(300∶0.5)-乙腈为流动相进行梯度洗脱,检测波长275 nm,流速1 mL·min-1,柱温25℃。结果:建立了10批紫草闪式提取醇提物的HPLC指纹色谱图,获得了13个共有峰,并指认了其中的3个色谱峰,4,6,10号分别被指认为左旋紫草素、乙酰紫草素和β,β'-二甲基丙烯酰阿卡宁。结论:该方法简便,精密度、重复性和稳定性良好,能有效控制紫草醇提物的质量,为进一步药效研究、制剂研发提供质量保证。
Objective： To establish the HPLC fingerprint of Arnebiae Radix smashing tissue extraction with ethanol and reflect the chemical information comprehensively and provide evidence for quality control. Method： HPLC was used on a Kromasil 100-5 C18 （4.6 mm×250 mm, 5 μm） by gradient elution with solvent system composed of water-formic acid （300：0.5）-acetonitrile, detection wavelength was 275 nm, the flow rate was 1 mL·min-1, the column temperature was set at 25 ℃. Result： HPLC fingerprint of Arnebiae Radix smashing tissue extraction with ethanol was established, which showed 13 characteristic peaks from 10 batches of crude drug. Among them, the No 4, 6, 10 peaks were respectively identified as shikonin, acetylshikonin, and β,β＇-dimethylacryl alkannin. Conclusion： The method is simple and has good precision, reproducibility and stability, and can be applied to the quality control the Arnebiae Radix smashing tissue extraction with ethanol, also provide a quality assurance for further pharmacological studies and preparation research.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第2期74-77,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京市中医药科技提升专项(KJTS2011-07)
