摘要
目的:观察蒿芩清胆汤对幽门螺杆菌相关性胃炎(HAG)小鼠血清白细胞介素-8(IL-8)水平及胃黏膜IL-8、核转录因子κB p65(NF-κB p65)的影响,探讨其对胃黏膜损伤修复的作用机制。方法:按体重、性别随机抽取10只小鼠作为空白对照组,其他小鼠采用幽门螺杆菌(HP)标准菌株(SS1)布氏肉汤液灌胃造模。造模成功后,50只模型小鼠按性别、体重随机分为5组,每组10只,即为模型组、丽珠维三联组(0.25 g·kg-1)、蒿芩清胆汤低、中、高剂量组(4,8,16 g·kg-1)。灌胃容积10mL·kg-1,空白对照组、模型组灌服等体积生理盐水,1次/d。2周后处死动物,观察各组小鼠胃黏膜病理学改变,酶联免疫法(ELISA)检测血清IL-8含量、免疫组化法检测胃黏膜IL-8,NF-κB p65的表达。结果:与模型组比较,蒿芩清胆汤能显著降低HAG小鼠胃黏膜炎症面积和平均灰度(P<0.01);蒿芩清胆汤明显降低HAG小鼠血清IL-8含量(P<0.01);蒿芩清胆汤能明显降低HAG小鼠胃黏膜IL-8,NF-κB p65的表达(P<0.05)。结论:蒿芩清胆汤对HAG小鼠胃黏膜损伤有明显修复作用,其机制可能与其抑制NF-κB p65的激活、下调IL-8的过度表达有关。
Objective： To investigate the effect of Haoqin Qingdan decoction on the content of interleukin 8（IL-8） in serum and expression of IL-8, neclear factor-κB p65（NF-κB p65） in Gastric mucosa in Helicobacter pylori-associated gastritis（HAG） mice and explore its therapeutic mechanism for Helicobacter pylori（HP） associated gastritis. Method： HP standard strains （SS1） brinell broth liquid was used to establish the HAG mice model. Sixty healthy BALB/c mice were randomly devided into six group （n=10, each）, normal control group（given with equal NS）, model group （given with equal NS）, livzon weisanlian group（0.25 g·kg-1）, Haoqin Qindan decoction （4,8,16 g·kg-1） group. The mice in every group were administrated with drugs by oral gavage once a day. All mice were killed two weeks later, the pathological changes of each group were observed by the microscope, the contents of IL-8 in serum were detected by enzyme-linked immunoassay and the expression of IL-8, NF-κB p65 in gastric mucosa tissue were detected by immunohistochemistry. Result： Compared with model group, Haoqin Qingdan decoction significantly reduced the inflammation area and average blackness of gastric mucosa in HAG mice （P〈0.01）;the expression of IL-8 in serum of Haoqin Qingdan decoction groups were significantly lower than that in model group （P〈0.01）;the expression of IL-8, NF-κB p65 in gastric mucosa of Haoqin Qingdan decoction groups were significantly lower than that in model group （P〈0.05）. Conclusion： Haoqin Qingdan decoction can plays a significant role in the repair of gastric mucosal injury in HAG mice, its therapeutic mechanism may be related to inhibiting the activation of NF-κB p65 and degrading the expression of IL-8 in the gastric mucosa and serum.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第2期152-156,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
四川省科技厅科技计划项目(2013JY0110)
