摘要
目的通过体外抗溃疡活性来评价延胡索总生物碱有机溶剂萃取的纯化工艺。方法采用酸性染料比色法,以延胡索总生物碱纯度为指标,考察大孔吸附树脂结合有机溶剂萃取纯化的总生物碱纯度,并采用MTT法测定GSE-1细胞相关活性评价,对总生物碱纯度和药效进行相关性分析。结果最佳纯化工艺为1 mol/L的HCl溶解树脂纯化物,1倍药液体积的三氯甲烷除杂,药液加氨水碱化,以1倍药液体积的有机溶剂,按照三氯甲烷-乙酸乙酯-正丁醇的顺序分段综合萃取,合并有机溶剂层。结论所建立的纯化工艺稳定可行,总生物碱纯度>90%,体外抗GSE-1溃疡量效关系明确。
AIM To optimize and screen the purity of total alkaloids from Corydalis Rhizoma with the aid of GSE-1. METHODS The purification of the total alkaloids was performed with organic solution and on macroporous adsorptive resins. Acid dye colorimetry was used to determine total alkaloids from R. Corydalis. MTT assay was used to evaluate the effect of depurantive total alkaloids on GSE-1 proliferation, and the correlation between the purity and potency of total alkaloids was studied. RESULTS The optimal extraction was that the purified extract was dissolved with 1 mol/L hydrochloric acid. And then the impurities were removed by double volume of chloro- form , ammonified, chloroform- acetoacetate- n-butanol extraction, three layers of organic solvent were collected and combined. CONCLUSION This process is reasonable and feasible the obtained purity of the total alkaloids is more than 90% , with a explicit connection resistance to ulcer of GSE-1 in vitro.
出处
《中成药》
CAS
CSCD
北大核心
2014年第1期56-61,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(81241111)
关键词
延胡索总生物碱
萃取
纯化工艺
抗溃疡
体外
total alkaloids from Corydalis Rhizoma
extraction
purification
anti-ulcer
in vitro
