摘要
【目的】建立一种敏感性好、简便快捷,适用于临床诊断、实验室检测和血清学调查的诊断技术,为规模化养猪场监控胸膜肺炎放线杆菌(APP)感染及净化猪传染性胸膜肺炎(PCP)提供技术支撑。【方法】以ApxIV重组蛋白为抗原,建立ApxIV-ELISA检测方法,经可行性、特异性、准确性、重复性检验后,对采自广西南宁周边地区随机抽取的未免疫APP疫苗育肥猪与经产母猪进行血清抗体检测。【结果】经试验证实,以ApxIV-ELISA检测APP具有可行性,且具有特异性良好、准确性较高、重复性较好的特点,其判断标准:S(样品孔OD630 nm)≥0.397为阳性,S<0.397为阴性。以ApxIV-ELISA检测的90份血清样品中有40份为阳性,阳性率为44.4%;其中经产母猪阳性血清33份,占总阳性血清的82.5%。【结论】广西南宁周边地区的养猪场已普遍存在APP感染,特别是经产母猪较严重。采用ApxIV-ELISA检测方法能及时鉴别诊断猪群的APP感染情况,且敏感性好、简便快捷,适用于临床诊断、实验室检测和血清学调查。
【Objective】In order to provide technical support for detecting and controlling Actinobacillus pleuropneumoniae （APP） and purifying Pleuropneumoniae in large-scale pig farms, we established a sensitive and convenient diagnostic technique for clinical diagnosis, laboratory testing and serological survey. 【Method】Setting up ApxIV-ELISA diagnostic method by the feasibility, specificity, accuracy and repeatability test with ApxIV recombinant protein, serum antibodies of no-immunized pleuropneumonia vaccine fattening pigs and multiparous sow, which came from pig farms in Nanning area of Guangxi, were detected. 【Result】The experiment verified that ApxIV-ELISA diagnostic method was feasible to detect APP. The method has good specificity, high accuracy, and good repeatability. The criteria for determination were as follows. When OD630 nm （Sample hole） was greater than or equal to 0.397, the serum was identified as positive. When OD630 nm was less than 0.397, the serum was identified as negative. In the ninety serum samples, forty serum samples were positive and the positive rate was 44.4%. In the forty positive serum samples, thirty-three serum samples came from the multiparous sow, and occupied 82.5% in the total positive serum. 【Conclusion】The pig farms infected with APP were prevalent, especially for multiparous sow in Nanning area of Guangxi. We could opportunely distinguish and diagnose cases of APP infection by ApxIV-ELISA method. The method is sensitive, convenient, and suitable for clinical diagnosis, laboratory testing and serological survey, hence it provides technical support for detecting and controlling Actinobacillus pleuropneumoniae （APP） and purifying Pleuropneumoniae in large-scale pig farms.
出处
《南方农业学报》
CAS
CSCD
北大核心
2013年第12期2098-2101,共4页
Journal of Southern Agriculture
基金
广西青年科学基金项目(GXNSFB018029)
