摘要
目的观察中药筋脉通胶囊对链尿佐菌素(STZ)诱导的糖尿病(DM)大鼠背根神经节(DRG)的核因子E2相关因子2(Nrf2)和血红素加氧酶-1(HO-1)的表达以及血浆一氧化碳(CO)含量的影响。方法腹腔内注射STZ诱导建立DM大鼠模型,随机分为模型组、筋脉通小、中和大剂量组及硫辛酸组,并设对照组。成模后每天1次灌胃给药,持续12周。电子Von Frey仪检测机械痛阈值,免疫组化法及Rt-PCR检测DRG的Nrf2和HO-1蛋白及mRNA表达,并检测血浆一氧化碳血红蛋白(COHb)含量。结果与对照组相比,糖尿病大鼠的机械痛阈值、血浆COHb含量以及DRG的Nrf2和HO-1蛋白及mRNA表达水平均明显下降(P<0.01);各治疗组的上述指标均显著回升(P<0.01或P<0.05)。在提高DRG的HO-1蛋白表达上,筋脉通中剂量组疗效显著优于硫辛酸组(P<0.05)。结论筋脉通胶囊可通过增强DRG的Nrf2、HO-1和CO表达来改善糖尿病大鼠的周围神经痛。
Objective To study the effects of Jinmaitong Capsule （JMT） on the expression of nuclear factor-E2-re- lated factor 2 （Nrf2） and haem oxygenase-1 （HO-1） and their mRNAs in dorsal root ganglion （DRG） and the lev- el of plasma carbon monoxide （CO） in STZ-induced diabetic rats. Methods STZ-induced diabetic rats were ran- domly divided into 5 groups including model group, low-dose JMT group, middle-dose JMT group, high-dose JMT groupand Thioctic Acid Capsule （TAC） group. Ten normal rats matching with weight and age served as the control group. All rats were treated by intragastric administration for 12 weeks and then culled. The mechanical withdrawal threshold was tested by electronic Von Frey instrument before death. The expressions of Nrf2 and HO-1 and their mRNAs in DRG were detected by SABC immunohistochemical method and real-time fluorogenetic quantitative PCRrespectively. The content of plasma COHb was also measured. Results Compared with the control group, the me- chanical withdrawal threshold, the content of plasma CO and the expressions of Nrf2 and HO-1 and their mRNAs in DRG of all the diabetic rats groups decreased significantly （P 〈 0. 01 ）. All the above-mentioned indexes in treatment groups raised strikingly （P 〈0. 05 or P 〈0. 01）. The middle-dose JMT group was more effective than the TAC group in enhancing the expression of HO-1 in DRG （P 〈0. 05）. Conclusions Jinmaitong Capsule can ameliorate the pe- ripheral neuropathic pain of STZ-DM rats by enhancing the expressions of Nrf2, HO-1 and CO in DRG.
出处
《基础医学与临床》
CSCD
北大核心
2014年第2期179-184,共6页
Basic and Clinical Medicine
基金
国家自然科学基金(81001580)
