摘要
目的制备小刺猴头菌发酵浸膏寡糖,并检测其对肠道菌群体外生长的影响。方法采用酶降解、酸降解两种方法制备小刺猴头菌发酵浸膏寡糖,通过葡聚糖凝胶层析法与荧光辅助糖电泳法(fluorophore assisted carbohydrate electrophoresis,FACE)进行分离分析,并观察不同降解条件产物对大肠埃希菌和鼠李糖乳杆菌体外生长的影响。结果最佳酶降解反应条件为料液比1∶45,反应温度50℃,反应时间5 h,选取还原糖得率低(21.67%)、中(32.33%)、高(36.11%)3组寡糖产物,分别命名为HFP-M1、HFP-M2、HFP-M3;最佳酸降解反应条件为反应温度25℃,pH 3,反应时间1 h,选取还原糖得率低(39.72%)、中(44.93%)、高(48.25%)3组寡糖产物,分别命名为HFP-S1、HFP-S2、HFP-S3;层析结果显示,酸降解寡糖比酶降解寡糖相对分子质量小;FACE分析显示,在32%的凝胶浓度下,酶降解寡糖可分离出较明显的条带,而酸降解寡糖条带不太清晰;两种方法降解获得的6种寡糖均能显著抑制大肠埃希菌的繁殖,促进鼠李糖乳杆菌的生长,且效果优于小刺猴头菌发酵浸膏多糖。结论采用酶降解和酸降解两种方法制备的小刺猴头菌发酵发酵浸膏寡糖对大肠埃希菌的繁殖具有抑制作用,对鼠李糖乳杆菌的生长均有促进作用。本实验为大分子小刺猴头菌发酵浸膏多糖及其降解产物应用于医药保健或功能性食品的开发提供了实验依据。
Objective To prepare H. caput-medusae（Bull, ：Fr） pers. （HFP） fermentation extract oligosaccharide and de- termine its effect on the growth of intestinal flora in vitro. Methods H. caput-medusae （Bull, ：Fr） pers. （HFP） fermenta- tion extract oligosaccbaride was prepared by enzyme degradation and acid degradation, separated by dextran gel chro- matography, and analyzed by fluorophore assisted carbohydrate electrophoresis（FACE）. The effects of degradation condi- tion on in vitro growths of E. coli and Lactobacillus rhamnosus were observed. Results The optimal solid-to-liquid ratio, temperature and reaction time for enzyme degradation were 1 ： 45, 50 ~C and 5 h respectively. Three groups of oligosac- charide with low （39. 72%）, moderate （44. 93%） and high （48. 25%） yields were named as HFP-S1, HFP-S2 and HFP-S3 respectively. Chromatography proved that the relative molecular mass of oligosaccharide degraded with acid was lower than that with enzyme. FACE showed obvious enzyme-degraded oligosaccharide band at a gel concentration of 32%, while the acid-degraded oligosaccharide band was unclear. All the six kinds of oligosaccharide degraded by two methods inhibited the propagation of E. coli significantly and promoted the growth of L. rhamnosus, and the effects were superior to those of HFP fermentation extract. Conlcusion H. caput-medusae （Bull; Fr）pen. （HFP）fermentation extract oligosaccharide pre- pared by enzyme and acid degradations inhibited the growth of E. coli while promoted that of L. rhamnosus, which provided an experimental basis for development of macromolecular H.caput-medusae （Bull;Fr） pers. （HFP） fermentation extract polysaccharides and their degradation products as medical or functional foods.
出处
《中国生物制品学杂志》
CAS
CSCD
2014年第1期120-124,共5页
Chinese Journal of Biologicals
基金
吉林省世行贷款农产品质量安全项目(2011-Y18)
关键词
小刺猴头菌发酵浸膏
多糖
寡糖
酶降解
酸降解
大肠埃希菌
鼠李糖乳杆菌
H. cap ut-medusae （Bull, ： Fr ） pe rs. （ HFP ） fermentation extract
Polysaccharide
Oligosaccharide
Enzyme degradation
Acid degradation
E. coli
Lactobacillus rhamnosus
